IMECH-IR  > 非线性力学国家重点实验室
Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing
Sun YN; Chen XD(陈晓东); Zhou XG; Zhu JB; Yu YD; Yu, YD (reprint author), Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, POB 912, Beijing 100083, Peoples R China.
Source PublicationLAB ON A CHIP
2015
Volume15Issue:11Pages:2429-2436
ISSN1473-0197
AbstractIn recent years, inkjet printing, as a new method to fabricate microdroplet microarrays, has been increasingly applied in the field of biochemical diagnostics. To further improve the general applicability of the inkjet printing technology in fabricating biochemical chips, in this work, we introduce a model to describe the multiple injection procedure implemented by the inkjet printing approach, with experimental verification. The multiple injection model demonstrates a new sequential inkjet printing method that generates picoliter-scale multicomponent droplet-in-oil arrays via multistep printing on uniform planar substrates. Based on our previous work on double-inkjet printing, this technique adapts the piezoelectric inkjet printing technology to fabricate an oil droplet array, into which multiple precise injections of secondary droplets with different compositions and volumes can be automatically printed in the required sequence, simultaneously addressing the evaporation issues associated with printing picoliter droplets without external assistance. In this paper, we first describe the theory and characterize the model, which account for the basic principles of sequential inkjet printing, as well as validate the design in terms of multiple injections, droplet fusion, and rapid mixing. The feasibility and effectiveness of the method are also demonstrated in a dual fluorescence assay and a beta-galactosidase enzyme inhibition assay. We believe that applying the sequential inkjet printing methodology in existing inkjet printing devices will enhance their use as universal diagnostic tools as well as accelerate the adoption of inkjet printing in multistep screening experiments.
DOI10.1039/c5lc00356c
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000354963400010
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
Funding OrganizationInstrument Development Project of the Chinese Academy of Sciences [61334008] ; National Natural Science Foundation of China [11402274] ; Scientific and Technological Innovation "Interdiscipline and Cooperation Team" Project of the Chinese Academy of Sciences
DepartmentLNM微纳米流体力学
Classification一类
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/54965
Collection非线性力学国家重点实验室
Corresponding AuthorYu, YD (reprint author), Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, POB 912, Beijing 100083, Peoples R China.
Recommended Citation
GB/T 7714
Sun YN,Chen XD,Zhou XG,et al. Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing[J]. LAB ON A CHIP,2015,15(11):2429-2436.
APA Sun YN,陈晓东,Zhou XG,Zhu JB,Yu YD,&Yu, YD .(2015).Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing.LAB ON A CHIP,15(11),2429-2436.
MLA Sun YN,et al."Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing".LAB ON A CHIP 15.11(2015):2429-2436.
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