Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry | |
Sun HL; Qi C(齐财); Niu Y(牛宇); Kang TF(康腾飞); Wei YX; Jin G(靳刚); Dong XZ; Wang CH; Zhu W; Zhu, W (reprint author), Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China. | |
发表期刊 | PLOS ONE |
2015-08-21 | |
卷号 | 10期号:8页码:e0136253 |
ISSN | 1932-6203 |
摘要 | Background Cytomegalovirus (CMV) is the most common infectious cause of mental disability in new-borns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. Methods An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A. Results Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL. Conclusions This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening. |
DOI | 10.1371/journal.pone.0136253 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000359926900090 |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
项目资助者 | Yantai Science and Technology Development Fund [2012128] ; Youth Backbone Teacher Fund of Binzhou Medical University ; National Natural Science Fund [81202516] ; Shandong Province Natural Science Fund [ZR2013HL039] ; Science and technology development plan of Shandong Academy of Medical Sciences [2014-08] ; Special Fund for Agro-scientific Research in the Public Interest [201303045] ; National Key Scientific Instrument and Equipment Development Project of China [2012YQ090197] ; National Basic Research Program (Project 973) of China [2009CB320300] ; National Basic Research Program of China [2009CB320302] ; National High Technology Research Development Program of China [2008AA02Z419] ; International Science & Technology Cooperation Program of China [2012DFG31880] ; Projects of Sino-Portugal ST Cooperation ; National Natural Science Foundation of China [21305147] ; Instrument Developing Project of the Chinese Academy of Sciences [KJCX2-YW-M04, KJCX2-YW-M03] |
课题组名称 | NML纳米生物光学 |
论文分区 | 二类/Q1 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://dspace.imech.ac.cn/handle/311007/55341 |
专题 | 微重力重点实验室 |
通讯作者 | Zhu, W (reprint author), Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China. |
推荐引用方式 GB/T 7714 | Sun HL,Qi C,Niu Y,et al. Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry[J]. PLOS ONE,2015,10,8,:e0136253. |
APA | Sun HL.,Qi C.,Niu Y.,Kang TF.,Wei YX.,...&Zhu, W .(2015).Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry.PLOS ONE,10(8),e0136253. |
MLA | Sun HL,et al."Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry".PLOS ONE 10.8(2015):e0136253. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
IMCAS-J2015-119.pdf(1747KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论