IMECH-IR  > 国家微重力实验室
Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry
Sun HL; Qi C(齐财); Niu Y(牛宇); Kang TF(康腾飞); Wei YX; Jin G(靳刚); Dong XZ; Wang CH; Zhu W; Zhu, W (reprint author), Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China.
Source PublicationPLOS ONE
2015-08-21
Volume10Issue:8Pages:e0136253
ISSN1932-6203
AbstractBackground Cytomegalovirus (CMV) is the most common infectious cause of mental disability in new-borns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. Methods An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A. Results Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL. Conclusions This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening.
DOI10.1371/journal.pone.0136253
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000359926900090
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
Funding OrganizationYantai Science and Technology Development Fund [2012128] ; Youth Backbone Teacher Fund of Binzhou Medical University ; National Natural Science Fund [81202516] ; Shandong Province Natural Science Fund [ZR2013HL039] ; Science and technology development plan of Shandong Academy of Medical Sciences [2014-08] ; Special Fund for Agro-scientific Research in the Public Interest [201303045] ; National Key Scientific Instrument and Equipment Development Project of China [2012YQ090197] ; National Basic Research Program (Project 973) of China [2009CB320300] ; National Basic Research Program of China [2009CB320302] ; National High Technology Research Development Program of China [2008AA02Z419] ; International Science & Technology Cooperation Program of China [2012DFG31880] ; Projects of Sino-Portugal ST Cooperation ; National Natural Science Foundation of China [21305147] ; Instrument Developing Project of the Chinese Academy of Sciences [KJCX2-YW-M04, KJCX2-YW-M03]
DepartmentNML纳米生物光学
Classification二类/Q1
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/55341
Collection国家微重力实验室
Corresponding AuthorZhu, W (reprint author), Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China.
Recommended Citation
GB/T 7714
Sun HL,Qi C,Niu Y,et al. Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry[J]. PLOS ONE,2015,10(8):e0136253.
APA Sun HL.,Qi C.,Niu Y.,Kang TF.,Wei YX.,...&Zhu, W .(2015).Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry.PLOS ONE,10(8),e0136253.
MLA Sun HL,et al."Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry".PLOS ONE 10.8(2015):e0136253.
Files in This Item: Download All
File Name/Size DocType Version Access License
IMCAS-J2015-119.pdf(1747KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun HL]'s Articles
[Qi C(齐财)]'s Articles
[Niu Y(牛宇)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun HL]'s Articles
[Qi C(齐财)]'s Articles
[Niu Y(牛宇)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun HL]'s Articles
[Qi C(齐财)]'s Articles
[Niu Y(牛宇)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: IMCAS-J2015-119.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.