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Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells
Wang CZ(王成之); Li N(李宁); Zhang C(张晨); Sun SJ(孙树津); Gao YX(高宇欣); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China.
Source PublicationMICROGRAVITY SCIENCE AND TECHNOLOGY
2015-09
Volume27Issue:6Pages:515-527
ISSN0938-0108
Abstract

Altered gravity, especially microgravity affects cellular functions of immune cells and can result in immune dysfunction for long-term, manned spaceflight and space exploration. The underlying mechanism, however, of sensing and responding to the gravity alteration is poorly understood. Here, a rotary cell culture system (RCCS) bioreactor was used to elucidate the effects of simulated microgravity on polymorphonuclear neutrophils (PMN)-like HL-60 cells. Alteration of cell morphology, up-regulation of (nitric oxide) NO production, enhancement of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein 1 (MCP-1) secretion, and diversity of cellular adhesion molecule expression were observed for the cells cultured in RCCS, leading to the up-regulated inflammatory immune responses and host defense. It was also indicated that such alterations in biological responses of PMNs mediated the reduced rolling velocity and decreased adhesion of PMN-like HL-60 cells on endothelial cells under shear flow. This work furthers the understandings in the effects and mechanism of microgravity on PMN functions, which are potentially helpful for optimizing the countermeasures to immune suppression in the future long-term, manned spaceflight.

KeywordHl-60 Pmn Effects Of Simulated Microgravity Rccs Flow Chamber
DOI10.1007/s12217-015-9473-6
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000366103800013
WOS KeywordShort-duration Spaceflight ; Nitric-oxide ; Endothelial-cells ; Parabolic Flight ; L-selectin ; Polymorphonuclear Leukocytes ; Growth-factor ; Bone-marrow ; Expression ; Gravity
WOS Research AreaEngineering ; Thermodynamics ; Mechanics
WOS SubjectEngineering, Aerospace ; Thermodynamics ; Mechanics
Funding OrganizationThis work was supported by National Key Basic Research Foundation of China grant 2011CB710904, National Natural Science Foundation of China grant 31110103918, Strategic Priority Research Program of Chinese Academy of Sciences grant XDA01030102, and National High Technology Research and Development Program of China grant 2011AA020109.
DepartmentNML分子-细胞生物力学与空间生命科学
Ranking1
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58369
Collection国家微重力实验室
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Wang CZ,Li N,Zhang C,et al. Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells[J]. MICROGRAVITY SCIENCE AND TECHNOLOGY,2015,27(6):515-527.
APA Wang CZ.,Li N.,Zhang C.,Sun SJ.,Gao YX.,...&Long, M .(2015).Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells.MICROGRAVITY SCIENCE AND TECHNOLOGY,27(6),515-527.
MLA Wang CZ,et al."Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells".MICROGRAVITY SCIENCE AND TECHNOLOGY 27.6(2015):515-527.
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