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Title:
Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells
Author: Wang, ChengZhi(王成之); Li N; Zhang C; Sun SJ(孙树津); Gao YX; Long M(龙勉)
Source: MICROGRAVITY SCIENCE AND TECHNOLOGY
Issued Date: 2015-09
Volume: 27, Issue:6, Pages:515-527
Abstract: Altered gravity, especially microgravity affects cellular functions of immune cells and can result in immune dysfunction for long-term, manned spaceflight and space exploration. The underlying mechanism, however, of sensing and responding to the gravity alteration is poorly understood. Here, a rotary cell culture system (RCCS) bioreactor was used to elucidate the effects of simulated microgravity on polymorphonuclear neutrophils (PMN)-like HL-60 cells. Alteration of cell morphology, up-regulation of (nitric oxide) NO production, enhancement of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein 1 (MCP-1) secretion, and diversity of cellular adhesion molecule expression were observed for the cells cultured in RCCS, leading to the up-regulated inflammatory immune responses and host defense. It was also indicated that such alterations in biological responses of PMNs mediated the reduced rolling velocity and decreased adhesion of PMN-like HL-60 cells on endothelial cells under shear flow. This work furthers the understandings in the effects and mechanism of microgravity on PMN functions, which are potentially helpful for optimizing the countermeasures to immune suppression in the future long-term, manned spaceflight.
Keyword: HL-60 ; PMN ; Effects of simulated microgravity ; RCCS ; Flow chamber
Language: 英语
Indexed Type: SCI ; EI
Corresponding Author: Long, M (reprint author), Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China.
Correspondent Email: mlong@imech.ac.cn
DOI: 10.1007/s12217-015-9473-6
Related URLs: 查看原文
DOC Type: 期刊论文
WOS Subject: Engineering, Aerospace ; Thermodynamics ; Mechanics
WOS Subject Extended: Engineering ; Thermodynamics ; Mechanics
WOS Keyword Plus: SHORT-DURATION SPACEFLIGHT ; NITRIC-OXIDE ; ENDOTHELIAL-CELLS ; PARABOLIC FLIGHT ; L-SELECTIN ; POLYMORPHONUCLEAR LEUKOCYTES ; GROWTH-FACTOR ; BONE-MARROW ; EXPRESSION ; GRAVITY
WOS ID: WOS:000366103800013
ISSN: 0938-0108
Funder: This work was supported by National Key Basic Research Foundation of China grant 2011CB710904, National Natural Science Foundation of China grant 31110103918, Strategic Priority Research Program of Chinese Academy of Sciences grant XDA01030102, and National High Technology Research and Development Program of China grant 2011AA020109.
Rank: [Wang, Chengzhi; Li, Ning; Zhang, Chen; Sun, Shujin; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China; [Wang, Chengzhi; Li, Ning; Zhang, Chen; Sun, Shujin; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China
Department: NML分子-细胞生物力学与空间生命科学
Classification: 二类/Q2
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/58369
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
Wang CZ,Li N,Zhang C,et al. Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells[J]. MICROGRAVITY SCIENCE AND TECHNOLOGY,2015-09-01,27(6):515-527.
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