IMECH-IR  > 非线性力学国家重点实验室
Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status
Qian Y; Zhang J; Hu QL; Xu M; Chen Y; Hu GQ(胡国庆); Zhao MR; Liu SJ; Liu, SJ (reprint author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China.
Source PublicationBIOMATERIALS
2015-11
Volume70Pages:12-22
ISSN0142-9612
AbstractSilver nanoparticles (nanosilver, AgNPs) have been shown to induce toxicity in vitro and in vivo; however, the molecular bases underlying the detrimental effects have not been thoroughly understood. Although there are numerous studies on its genotoxicity, only a few studies have investigated the epigenetic changes, even less on the changes of histone modifications by AgNPs. In the current study, we probed the AgNP-induced alterations to histone methylation that could be responsible for globin reduction in erythroid cells. AgNP treatment caused a significant reduction of global methylation level for histone 3 (H3) in erythroid MEL cells at sublethal concentrations, devoid of oxidative stress. The ChIP-PCR analyses demonstrated that methylation of H3 at lysine (Lys) 4 (H3K4) and Lys 79 (H3K79) on the beta-globin locus was greatly reduced. The reduction in methylation could be attributed to decreased histone methyltransferase DOT-1L and MLL levels as well as the direct binding between AgNPs to H3/H4 that provide steric hindrance to prevent methylation as predicted by the all-atom molecular dynamics simulations. This direct interaction was further proved by AgNP-mediated pull-down assay and immunoprecipitation assay. These changes, together with decreased RNA polymerase II activity and chromatin binding at this locus, resulted in decreased hemoglobin production. By contrast, Ag ion-treated cells showed no alterations in histone methylation level. Taken together, these results showed a novel finding in which AgNPs could alter the methylation status of histone. Our study therefore opens a new avenue to study the biological effects of AgNPs at sublethal concentrations from the perspective of epigenetic mechanisms. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordNanosilver Epigenetics Transcription Hemoglobin Histone Methylation
DOI10.1016/j.biomaterials.2015.08.015
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000362610600002
WOS KeywordBETA-GLOBIN GENE ; MOLECULAR-DYNAMICS ; ENGINEERED NANOMATERIALS ; DOT1L METHYLTRANSFERASE ; CHROMATIN MODIFICATIONS ; EPIGENETIC REGULATION ; GOLD NANOPARTICLES ; H3K79 METHYLATION ; IN-VIVO ; MECHANISMS
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
Funding OrganizationThis work was supported by a grant under the National "973" program (grant number: 2014CB932000), the Strategic Priority Research Program of the Chinese Academy of Sciences (grant number. XDB14000000) and grants from the National Natural Science Foundation of China (grant numbers: 21425731, 21377159, 21321004, 21207152, 21407169). We thank all the laboratory members for their great assistance with experiments and reagents.
DepartmentLNM微纳米流体力学
Classification一类
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58399
Collection非线性力学国家重点实验室
Corresponding AuthorLiu, SJ (reprint author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China.
Recommended Citation
GB/T 7714
Qian Y,Zhang J,Hu QL,et al. Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status[J]. BIOMATERIALS,2015,70:12-22.
APA Qian Y.,Zhang J.,Hu QL.,Xu M.,Chen Y.,...&Liu, SJ .(2015).Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status.BIOMATERIALS,70,12-22.
MLA Qian Y,et al."Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status".BIOMATERIALS 70(2015):12-22.
Files in This Item: Download All
File Name/Size DocType Version Access License
J2015-042.pdf(2005KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qian Y]'s Articles
[Zhang J]'s Articles
[Hu QL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qian Y]'s Articles
[Zhang J]'s Articles
[Hu QL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qian Y]'s Articles
[Zhang J]'s Articles
[Hu QL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: J2015-042.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.