IMECH-IR  > 力学所知识产出(1956-2008)
A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E
Li AL; Li HW; Jin G(靳刚); Xiu RJ; Xiu, RJ (reprint author), Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China.
Source PublicationCLINICAL HEMORHEOLOGY AND MICROCIRCULATION
2003
Volume29Issue:3-4Pages:383-390
ISSN1386-0291
AbstractThis study was designed to comprehensively analyze the differential expression of proteins from human umbilical vein endothelial cells (HUVECs) exposed to tumor conditioned medium (TCM) and to identify the key regulator in the cell cycle progression. The HUVECs were exposed to TCM from breast carcinoma cell line MDA-MB-231, then their cell cycle distribution was measured by flow cytometer (FCM). The role of protein in cell cycle progression was detected via two-dimensional polyacrylamide gel electrophoresis (2-DE) and western blotting. Following the stimulation of TCM, HUVECs showed a more cells in the S phase than did the negative control group (ECGF-free medium with 20% FBS), but the HUVECs' level was similar to the positive control group (medium with 25 mug/ml ECGF and 20% FBS). Increased expression of cyclin D-1/E and some changes in other related proteins occurred after incubation with TCM. From our results, we can conclude that breast carcinoma cell line MDA-MB-231 may secrete soluble pro-angiogenic factors that induce the HUVEC angiogenic switch, during which the expression of cell cycle regulator cyclin D-1/E increases and related proteins play an important role in this process.
KeywordTumor Conditioned Medium Endothelium Cell Cycle 2-de Western Blot
Indexed BySCI
Language英语
WOS IDWOS:000188240800032
WOS KeywordGROWTH-FACTOR ; CANCER-CELLS ; AGENT TNP-470 ; IN-VITRO ; ANGIOGENESIS ; ANTISENSE ; D1 ; INHIBITION ; P21(WAF1/CIP1) ; TUMORIGENICITY
WOS Research AreaHematology ; Cardiovascular System & Cardiology
WOS SubjectHematology ; Peripheral Vascular Disease
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58477
Collection力学所知识产出(1956-2008)
Corresponding AuthorXiu, RJ (reprint author), Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China.
Recommended Citation
GB/T 7714
Li AL,Li HW,Jin G,et al. A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2003,29(3-4):383-390.
APA Li AL,Li HW,靳刚,Xiu RJ,&Xiu, RJ .(2003).A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,29(3-4),383-390.
MLA Li AL,et al."A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION 29.3-4(2003):383-390.
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