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Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer
Huang CH; Li JX; Tang Y; Wang CX; Hou CJ; Huo DQ; Chen YY(陈艳艳); Jin G(靳刚); Jin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China.
Source PublicationMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
2011
Volume31Issue:7Pages:1609-1613
ISSN0928-4931
AbstractIn this study, a practicable method for the detection of Riemerella anatipestifer (R. anatipestifer) using biosensor based on imaging ellipsometry (BIE) is described. The method is performed by immobilizing anti-R. anatipestifer egg yolk immunoglubilin (IgY) onto modified chemistry surface to form sensing layer. Antigen captured by sensing layer can then be quantitatively measured through imaging ellipsometry in grayscale format. The results demonstrate that it can detect R. anatipestifer as low as 5.2 x 10(3) CFU/mL. Furthermore, the proposed method can realize specific discrimination for multiple serotypes of 1,2, 4, and 14 of R. anatipestifer. It has the advantages of rapid, simple, high sensitivity and low cost. (C) 2011 Elsevier B.V. All rights reserved.
KeywordBiosensor Imaging Ellipsometry Riemerella Anatipestifer Detection
DOI10.1016/j.msec.2011.05.018
Indexed BySCI ; EI
Language英语
WOS IDWOS:000295953900051
WOS KeywordPASTEURELLA-ANATIPESTIFER ; ESCHERICHIA-COLI ; IMMUNOSENSOR ; DUCKS ; VISUALIZATION ; INFECTION
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
Funding OrganizationNatural Science Foundation [20845003, 10804083] ; Ministry of Science and Technology of China [2008AA02Z419] ; Chinese Academy of Sciences [KJCX2.YW.M04] ; Natural Science Foundation of Chongqing [CSTC2008 BB1245] ; Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences
DepartmentNML纳米生物光学
Classification一类
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58582
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Huang CH,Li JX,Tang Y,et al. Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2011,31(7):1609-1613.
APA Huang CH.,Li JX.,Tang Y.,Wang CX.,Hou CJ.,...&Jin, G .(2011).Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,31(7),1609-1613.
MLA Huang CH,et al."Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 31.7(2011):1609-1613.
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