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Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry
Huang CH; Li JX; Tang Y; Chen YY(陈艳艳); Jin G(靳刚); Jin, G (reprint author), CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China.
Source PublicationCURRENT APPLIED PHYSICS
2011
Volume11Issue:3Pages:353-357
ISSN1567-1739
AbstractA novel method for the detection of duck hepatitis virus serotype1 (DHV1) using a biosensor based on imaging ellipsometry (IE) is developed. Polyclonal antibodies against DHV1 were immobilized on silicon wafers through protein A and used to capture virus particles in sample. Signals on silicon surface were acquired directly in grayscale format on an imaging ellipsometry image. This study demonstrated that the assay is rapid and accurate for detection of duck hepatitis virus. Compared with traditional methods, this technique not only has high sensitivity as low as 8 x 10(-9.5) LD(50)/ml, but can also simultaneously perform multiple serotype identification of DHV1, DHV1-g and DHV1-s. These results suggest that this biosensor might be a practicable diagnostic tool for duck hepatitis virus detection. (C) 2010 Elsevier B.V. All rights reserved.
KeywordBiosensor Imaging Ellipsometry Duck Hepatitis Virus Detection
DOI10.1016/j.cap.2010.08.004
Indexed BySCI ; EI
Language英语
WOS IDWOS:000288183300017
WOS KeywordVISUALIZATION
WOS Research AreaMaterials Science ; Physics
WOS SubjectMaterials Science, Multidisciplinary ; Physics, Applied
Funding OrganizationMinistry of Science and Technology of China [2008AA02Z419] ; Natural Science Foundation of China [10804083, 20845003] ; Chinese Academy of Sciences [KJCX2.YW.M04] ; Key Laboratory of Microgravity, Institute of Mechanics, CAS ; southwest University, Chongqing, China
DepartmentNML纳米生物光学
Classification二类
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58588
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China.
Recommended Citation
GB/T 7714
Huang CH,Li JX,Tang Y,et al. Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry[J]. CURRENT APPLIED PHYSICS,2011,11(3):353-357.
APA Huang CH,Li JX,Tang Y,陈艳艳,靳刚,&Jin, G .(2011).Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry.CURRENT APPLIED PHYSICS,11(3),353-357.
MLA Huang CH,et al."Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry".CURRENT APPLIED PHYSICS 11.3(2011):353-357.
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