Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry

doi:10.1016/j.cap.2010.08.004

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	Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry
	Huang CH; Li JX; Tang Y; Chen YY(陈艳艳); Jin G(靳刚); Jin, G (reprint author), CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China.
发表期刊	CURRENT APPLIED PHYSICS
	2011
卷号	11 期号:3 页码:353-357
ISSN	1567-1739
摘要	A novel method for the detection of duck hepatitis virus serotype1 (DHV1) using a biosensor based on imaging ellipsometry (IE) is developed. Polyclonal antibodies against DHV1 were immobilized on silicon wafers through protein A and used to capture virus particles in sample. Signals on silicon surface were acquired directly in grayscale format on an imaging ellipsometry image. This study demonstrated that the assay is rapid and accurate for detection of duck hepatitis virus. Compared with traditional methods, this technique not only has high sensitivity as low as 8 x 10(-9.5) LD(50)/ml, but can also simultaneously perform multiple serotype identification of DHV1, DHV1-g and DHV1-s. These results suggest that this biosensor might be a practicable diagnostic tool for duck hepatitis virus detection. (C) 2010 Elsevier B.V. All rights reserved.
关键词	Biosensor Imaging Ellipsometry Duck Hepatitis Virus Detection
DOI	10.1016/j.cap.2010.08.004
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000288183300017
关键词[WOS]	VISUALIZATION
WOS研究方向	Materials Science ; Physics
WOS类目	Materials Science, Multidisciplinary ; Physics, Applied
项目资助者	Ministry of Science and Technology of China [2008AA02Z419] ; Natural Science Foundation of China [10804083, 20845003] ; Chinese Academy of Sciences [KJCX2.YW.M04] ; Key Laboratory of Microgravity, Institute of Mechanics, CAS ; southwest University, Chongqing, China
课题组名称	NML纳米生物光学
论文分区	二类
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/58588
专题	微重力重点实验室
通讯作者	Jin, G (reprint author), CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China.
推荐引用方式 GB/T 7714	Huang CH,Li JX,Tang Y,et al. Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry[J]. CURRENT APPLIED PHYSICS,2011,11,3,:353-357.
APA	Huang CH,Li JX,Tang Y,陈艳艳,靳刚,&Jin, G .(2011).Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry.CURRENT APPLIED PHYSICS,11(3),353-357.
MLA	Huang CH,et al."Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry".CURRENT APPLIED PHYSICS 11.3(2011):353-357.

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