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Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability
Zhang MH; Lv SQ(吕守芹); Li GW; Mao ZL; Yu X; Sun WN; Tang ZC; Long M(龙勉); Su WA; Long, MA (reprint author), 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
Source PublicationJOURNAL OF BIOLOGICAL CHEMISTRY
2010
Volume285Issue:53Pages:41982-41992
ISSN0021-9258
AbstractMolecular selection, ion exclusion, and water permeation are well known regulatory mechanisms in aquaporin. Water permeability was found to be diverse in different subgroups of plasma membrane intrinsic proteins (PIPs), even though the residues surrounding the water holes remained the same across the subgroups. Upon homology modeling and structural comparison, a conserved Ala/Ile(Val) residue difference was identified in helix 2 that affected the conformation of the NPA region and consequently influenced the water permeability. The residue difference was found to be conservative within the two subgroups of PIPs in rice as well as in other plants. Functional tests further confirmed the prediction via site-directed mutagenesis where replacement of Ala(103) or Ala(102) in respective OsPIP1; 1 or OsPIP1; 3 with Val yielded 7.0- and 2.2-fold increases in water transportation, and substitution of Ile(98) or Val(95) in respective OsPIP2; 3 or OsPIP2; 7 with Ala resulted in 73 or 52% reduction of water transportation. Based on structural analyses and molecular dynamics simulations, we proposed that the difference in water permeability was attributed to the orientation variations of helix 2 that modified water-water and water-protein interactions.
DOI10.1074/jbc.M110.101790
Indexed BySCI ; EI
Language英语
WOS IDWOS:000285622600084
WOS KeywordMOLECULAR-DYNAMICS ; AQUAPORIN NTAQP1 ; CHANNEL ACTIVITY ; STRUCTURAL BASIS ; TRANSPORT ; FAMILY ; ARABIDOPSIS ; SELECTIVITY ; PERMEATION ; GLYCEROL
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
Funding OrganizationNational Natural Science Foundation of China [30670172, 30730032, 10332060] ; National Key Basic Research Foundation of China [2006CB910303] ; Chinese Academy of Science [KSCX2-YW-N-011, KJCX2-YW-L08] ; National High Technology Research and Development Program of China [2007AA100603, 2007AA02Z306]
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58592
Collection国家微重力实验室
Corresponding AuthorLong, MA (reprint author), 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Zhang MH,Lv SQ,Li GW,et al. Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2010,285(53):41982-41992.
APA Zhang MH.,吕守芹.,Li GW.,Mao ZL.,Yu X.,...&Long, MA .(2010).Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability.JOURNAL OF BIOLOGICAL CHEMISTRY,285(53),41982-41992.
MLA Zhang MH,et al."Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability".JOURNAL OF BIOLOGICAL CHEMISTRY 285.53(2010):41982-41992.
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