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Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces
Almeida I; Marques JT; Liu W; Niu Y; de Almeida RFM; Jin G(靳刚); Viana AS; Viana, AS (reprint author), Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, Edificio C8, P-1749016 Lisbon, Portugal.
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
2015-12-01
Volume136Pages:997-1003
ISSN0927-7765
AbstractIn this work, a simple yet robust method to prepare lipid-based biosensing interfaces on gold using common lipids (a phospholipid and cholesterol) and an alkanethiol is reported. The lipids were carefully chosen to tailor the biophysical properties of the bilayer. The simplicity of the method relies on the incorporation of a small percentage of decanethiol in the lipid vesicles for a direct formation of a thiol-linked supported lipid bilayer, which is advantageous in several respects. It prevents the use of specially synthesized thiolipids and preserves the natural fluidity and dynamics of the lipids. As a consequence the whole arrangement is extremely stable regarding ionic strength changes and solution flow during surface plasmon resonance experiments. Moreover, we show that this interface is very effective on suppressing the nonspecific adsorption of proteins on the surface, and enables the covalent attachment of the recognition antibody. The subsequent detection of specific interaction toward antigen was monitored in real-time by SPR and confirmed by ellipsometric measurements. This lipid-based biosensing platform is versatile and can be adapted to the biorecognition reaction of interest. (C) 2015 Elsevier B.V. All rights reserved.
KeywordPlanar Hybrid Supported Lipid Bilayers Thiol-gold Linkages Antibody Covalent Coupling Inhibition Of Nonspecific Adsorption Immunosensors Surface Plasmon Resonance
DOI10.1016/j.colsurfb.2015.10.048
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000367408100121
WOS KeywordSURFACE-PLASMON RESONANCE ; BILAYER-LIPID MEMBRANES ; PHOSPHOLIPID-BILAYERS ; PHASE-EQUILIBRIA ; MODIFIED GOLD ; CHOLESTEROL ; IMMOBILIZATION ; MONOLAYERS ; RAFTS ; PHOSPHATIDYLCHOLINE
WOS Research AreaBiophysics ; Chemistry ; Materials Science
WOS SubjectBiophysics ; Chemistry, Physical ; Materials Science, Biomaterials
Funding OrganizationThis work was financed by the 7th Sino-Portugal Scientific and Technological Cooperation of 2013-2015 and by Portuguese national funds through FCT: Ph.D. fellowships: SFRH/BD/64442/2009, SFRH/BD/70673/2010 grants PEst OE/QUI/UI0612/2013 and PEst 2015-2020 (UID/Multi/00612/2013), and IF 2012/2013 initiatives (POPH, Fundo Social Europeu).
DepartmentNML纳米生物光学
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58654
Collection国家微重力实验室
Corresponding AuthorViana, AS (reprint author), Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, Edificio C8, P-1749016 Lisbon, Portugal.
Recommended Citation
GB/T 7714
Almeida I,Marques JT,Liu W,et al. Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2015,136:997-1003.
APA Almeida I.,Marques JT.,Liu W.,Niu Y.,de Almeida RFM.,...&Viana, AS .(2015).Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces.COLLOIDS AND SURFACES B-BIOINTERFACES,136,997-1003.
MLA Almeida I,et al."Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces".COLLOIDS AND SURFACES B-BIOINTERFACES 136(2015):997-1003.
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