|Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces|
|Almeida I; Marques JT; Liu W; Niu Y; de Almeida RFM; Jin G(靳刚); Viana AS; Viana, AS (reprint author), Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, Edificio C8, P-1749016 Lisbon, Portugal.
|Source Publication||COLLOIDS AND SURFACES B-BIOINTERFACES
|Abstract||In this work, a simple yet robust method to prepare lipid-based biosensing interfaces on gold using common lipids (a phospholipid and cholesterol) and an alkanethiol is reported. The lipids were carefully chosen to tailor the biophysical properties of the bilayer. The simplicity of the method relies on the incorporation of a small percentage of decanethiol in the lipid vesicles for a direct formation of a thiol-linked supported lipid bilayer, which is advantageous in several respects. It prevents the use of specially synthesized thiolipids and preserves the natural fluidity and dynamics of the lipids. As a consequence the whole arrangement is extremely stable regarding ionic strength changes and solution flow during surface plasmon resonance experiments. Moreover, we show that this interface is very effective on suppressing the nonspecific adsorption of proteins on the surface, and enables the covalent attachment of the recognition antibody. The subsequent detection of specific interaction toward antigen was monitored in real-time by SPR and confirmed by ellipsometric measurements. This lipid-based biosensing platform is versatile and can be adapted to the biorecognition reaction of interest. (C) 2015 Elsevier B.V. All rights reserved.|
|Keyword||Planar Hybrid Supported Lipid Bilayers
Antibody Covalent Coupling
Inhibition Of Nonspecific Adsorption
Surface Plasmon Resonance
|WOS Keyword||SURFACE-PLASMON RESONANCE
; BILAYER-LIPID MEMBRANES
; MODIFIED GOLD
|WOS Research Area||Biophysics
; Materials Science
; Chemistry, Physical
; Materials Science, Biomaterials
|Funding Organization||This work was financed by the 7th Sino-Portugal Scientific and Technological Cooperation of 2013-2015 and by Portuguese national funds through FCT: Ph.D. fellowships: SFRH/BD/64442/2009, SFRH/BD/70673/2010 grants PEst OE/QUI/UI0612/2013 and PEst 2015-2020 (UID/Multi/00612/2013), and IF 2012/2013 initiatives (POPH, Fundo Social Europeu).
|Corresponding Author||Viana, AS (reprint author), Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, Edificio C8, P-1749016 Lisbon, Portugal.|
Almeida I,Marques JT,Liu W,et al. Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2015,136:997-1003.
Almeida I.,Marques JT.,Liu W.,Niu Y.,de Almeida RFM.,...&Viana, AS .(2015).Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces.COLLOIDS AND SURFACES B-BIOINTERFACES,136,997-1003.
Almeida I,et al."Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces".COLLOIDS AND SURFACES B-BIOINTERFACES 136(2015):997-1003.