IMECH-IR  > 非线性力学国家重点实验室
A pilot study of creating keratoconus model by collagenase type II
Qiao J; Yan XM; Song WJ; Tang Y; Rong B; Li HL; Yang SL; Yin YQ(印亚奇); Wu Y
Source PublicationINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2015
Conference NameAnnual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO)
Conference DateMAY 03-07, 2015
Conference PlaceDenver, CO
Abstract

PurposeTo set up an experimental animal model of keratoconus using collagenase type II and evaluate corneal curvature changes.

Methods20 mortem New Zealand white rabbit corneas were divided into 4 groups: group 1(15 mg/ml collagenase type II with 15% dextran, N=5), group 2(10 mg/ml collagenase type II with 15% dextran, N=5), group 3(5 mg/ml collagenase type II with 15% dextran, N=5) and group 4(the control group,15% dextran, N=5). After epithelial debridement, corneas were mounted and pressured on artificial anterior chambers,then different kinds of solution was applied to corneas for 1 hour. Corneal curvature was measured before and after collagenase exposure at different intraocular pressure levels(15mmHg, 30mmHg,45mmHg). The results were analyzed statistically.

ResultsAfter exposure, changes of Kmean in group 1,group 2,group 3 and group 4 were 6.33±4.05D, 0.93±1.13D, 0.67±1.66D and -0.26±0.77D respectively at 15mmHg; 5.36±2.39D, 1.49±1.70D, 1.37±2.07D and -0.77±1.11D respectively at 30mmHg; 7.29±3.39D, 2.41±2.37D, 1.47±2.63D and -0.56±1.35D respectively at 45mmHg. Compared to group 4, a statistically significant increase in Kmean across all the intraocular pressure levels was seen in group 1 (p<0.05).However, group 2, group3 and group 4 were not significantly different from group 4(p>0.05).

Conclusions15 mg/ml collagenase type II could induce significant increase in corneal curvature, which might be considered as a method of building the rabbit model of keratoconus.

WOS IDWOS:000362882203022
Indexed BySCI ; CPCI-S
Language英语
Citation statistics
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58855
Collection非线性力学国家重点实验室
Recommended Citation
GB/T 7714
Qiao J,Yan XM,Song WJ,et al. A pilot study of creating keratoconus model by collagenase type II[C]INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2015.
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