Interlamellar cohesion after collagenase type II exposure in rabbit cornea | |
Yan XM; Qiao J; Song WJ; Tang Y; Li HL; Rong B; Yang SL; Yin YQ(印亚奇); Wu Y | |
会议录名称 | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE |
2015 | |
会议名称 | Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) |
会议日期 | MAY 03-07, 2015 |
会议地点 | Denver, CO |
摘要 | Purpose: Collagenase could be considered as a method for generating animal model of keratoconus. The authors aimed to evaluate the impact of collagenase on the interlamellar cohesive force of rabbit corneas. Methods: 20 post mortem New Zealand white rabbit corneas were divided into 4 groups: group 1(15 mg/ml collagenase type II with 15% dextran, N=5), group 2(10 mg/ml collagenase type II with 15% dextran, N=5),group 3(5 mg/ml collagenase type II with 15% dextran, N=5) and group 4(the control group,15% dextran, N=5). After removing epithelium and measuring the corneal thickness, 9*4mm corneal strips were incised and interlamellar cohesive force at 50% depth of stroma was measured with a microcomputer-controlled biomaterial testing machine. Results: The mean interlamellar cohesive force of group 1,group 2,group 3 and group 4 was 0.225 N/mm,0.217 N/mm,0.199 N/mm,and 0.211 N/mm respectively; without statistically significant differences. Light microscopy showed stromal tissue became less tight after collagenase exposure. Conclusions: Collagenase exposure does not decrease the interlamellar cohesive force in rabbit corneas, indicating that there is no significant effect of collagenase on interlamellar cohesion. Histopathology revealed less tight stroma after collagenase exposure due to its collagen digestion effect. |
WOS记录号 | WOS:000362882203021 |
收录类别 | SCI ; CPCI-S |
语种 | 英语 |
引用统计 | |
文献类型 | 会议论文 |
条目标识符 | http://dspace.imech.ac.cn/handle/311007/58856 |
专题 | 非线性力学国家重点实验室 |
推荐引用方式 GB/T 7714 | Yan XM,Qiao J,Song WJ,et al. Interlamellar cohesion after collagenase type II exposure in rabbit cornea[C]INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2015. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论