IMECH-IR  > 非线性力学国家重点实验室
Interlamellar cohesion after collagenase type II exposure in rabbit cornea
Yan XM; Qiao J; Song WJ; Tang Y; Li HL; Rong B; Yang SL; Yin YQ(印亚奇); Wu Y
Source PublicationINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2015
Conference NameAnnual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO)
Conference DateMAY 03-07, 2015
Conference PlaceDenver, CO
Abstract

PurposeCollagenase could be considered as a method for generating animal model of keratoconus. The authors aimed to evaluate the impact of collagenase on the interlamellar cohesive force of rabbit corneas.

Methods20 post mortem New Zealand white rabbit corneas were divided into 4 groups: group 1(15 mg/ml collagenase type II with 15% dextran, N=5), group 2(10 mg/ml collagenase type II with 15% dextran, N=5),group 3(5 mg/ml collagenase type II with 15% dextran, N=5) and group 4(the control group,15% dextran, N=5). After removing epithelium and measuring the corneal thickness, 9*4mm corneal strips were incised and interlamellar cohesive force at 50% depth of stroma was measured with a microcomputer-controlled biomaterial testing machine.

ResultsThe mean interlamellar cohesive force of group 1,group 2,group 3 and group 4 was 0.225 N/mm,0.217 N/mm,0.199 N/mm,and 0.211 N/mm respectively; without statistically significant differences. Light microscopy showed stromal tissue became less tight after collagenase exposure.

ConclusionsCollagenase exposure does not decrease the interlamellar cohesive force in rabbit corneas, indicating that there is no significant effect of collagenase on interlamellar cohesion. Histopathology revealed less tight stroma after collagenase exposure due to its collagen digestion effect.

WOS IDWOS:000362882203021
Indexed BySCI ; CPCI-S
Language英语
Citation statistics
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58856
Collection非线性力学国家重点实验室
Recommended Citation
GB/T 7714
Yan XM,Qiao J,Song WJ,et al. Interlamellar cohesion after collagenase type II exposure in rabbit cornea[C]INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2015.
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