7th International Conference on Nanochannels, Microchannels and Minichannels
JUN 22-24, 2009
Pohang, SOUTH KOREA
A capillary electrophoresis microchip system with a confocal LIF detector was successfully constructed. RBPhOH, a new fluorescence dye synthesized from rhodamine B, was first investigated in a glass microchip for the selective and sensitive determination of copper ion by LIP method. The influences of the pH value, concentration of running buffer, detection voltage, and sample loading time on the response of LIP detector were studied. RBPhOH dissolved in 20 mM pH 6.0 Tris containing 10% acetonitrile could detect copper ions with a detection limit of 1.34x10-7 mol L(-1) (S/N=3), an order of magnitude lower than those of conductivity detectors.