IMECH-IR  > 力学所知识产出(1956-2008)
Protein competitive adsorption and its effect on cell adhesion
Ying PQ; Jin G(靳刚); Tao ZL; Ying, PQ (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
Source PublicationIEEE-EMBS ASIA PACIFIC CONFERENCE ON BIOMEDICAL ENGINEERING - PROCEEDINGS, PTS 1 & 2
2000
Conference NameIEEE-EMBS Asia-Pacific Conference on Biomedical Engineering
Conference DateSEP 26-28, 2000
Conference PlaceHANGZHOU, PEOPLES R CHINA
AbstractThe purpose of this research was to investigate the competitive adsorption of bovine serum albumin (BSA) and collagen on various hydrophilicity surfaces with Imaging Ellipsometry and its effects on cell adhesion. Silicon surfaces with various hydrophilicity were incubated with the binary protein solution and an anti-BSA solution in order. By measuring the thickness distribution of the protein layers with Imaging Ellipsometry, protein competitive adsorption on substrata could be deduced. Substrata coated with protein were incubated in NIH3T3 fibroblasts or ROS 17/2.8 cells suspension The results showed that (1) in the binary protein solution of BSA and collagen, BSA preferentially adsorbed onto hydrophobic surfaces, but collagen on hydrophilic surfaces. (2) NIH3T3 fibroblasts adhered mostly and spread well on hydrophilic surface but hardly spread on hydrophobic surfaces conditioned with dimethyldichlorosilane (DMS). But it was reversed for ROS cell adhesion on hydrophobic surfaces conditioned with octadecyltrichlorosilane (OTS) and hydrophilic surfaces showing that cell adhesion was influenced by not only protein competitive adsorption but also cell type.
WOS IDWOS:000165806500270
ISBN7-5062-4828-X
Indexed ByCPCI-S
Language英语
Citation statistics
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/58960
Collection力学所知识产出(1956-2008)
Corresponding AuthorYing, PQ (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
Recommended Citation
GB/T 7714
Ying PQ,Jin G,Tao ZL,et al. Protein competitive adsorption and its effect on cell adhesion[C]IEEE-EMBS ASIA PACIFIC CONFERENCE ON BIOMEDICAL ENGINEERING - PROCEEDINGS, PTS 1 & 2,2000.
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