| Cross-Interface Emulsification for Generating Size-Tunable Droplets |
| Xu P; Zheng X(郑旭); Tao Y ; Du WB; Du, WB (reprint author), Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China.
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| 发表期刊 | ANALYTICAL CHEMISTRY
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| 2016
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| 卷号 | 88期号:6页码:3171-3177 |
| ISSN | 0003-2700
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| 摘要 | We report cross-interface emulsification (XiE), a pimple method for the generation of monodisperse droplets of controllable volumes from picoliter to nanoliter. A device is set up in which a fused-silica capillary Is vibrating across the surface of the continuous phase (mineral oil) in a reservoir, and the flow of the dispersed phase (aqueous solution) in the capillary is segmented into monodisperse droplets at the air/oil interface. We, find that the volume of droplets is mainly dominated by the flow rate and vibrating frequency and not significantly influenced by other factors,, such as the viscosity of the continuous phase and dispersed phase, the inner diameter of the capillary (20-100 mu m), Or the shape of the tip (tapered or flat). These features reflect high robustness, flexibility, and precision of XiE, for on-demand volume control of droplets. The droplets automatically assemble into planar monolayer droplet arrays (PMDA) in flat-bottomed microwells of 96-well plates, offering excellent convenience for imaging of droplets. As a representative application, we carry out digital loop-mediated isothermal amplification using PMDAs with multivolume droplets for the absolute quantification of nucleic acids. Our results demonstrate that XiE is simple and controllable for the production of monodisperse size-tunable droplets, and it offers opportunities for common laboratories, even without microfabrication facilities, to perform digital quantification, single cell analysis, and other biochemical assays with high throughput. |
| DOI | 10.1021/acs.analchem.5b04510
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| URL | 查看原文
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| 收录类别 | SCI
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| 语种 | 英语
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| WOS记录号 | WOS:000372391500028
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| WOS研究方向 | Chemistry
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| WOS类目 | Chemistry, Analytical
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| 项目资助者 | This work was supported by the program of China Ocean Mineral Resources R&D Association (DY125-15-R-02), the Strategic Priority Research Program of Chinese Academy of Sciences (XDB15040102), the National Natural Science Foundation of China (21205134, 31470221, 11202219, and 11572335), the National High Technology Research and Development Program of China (2012AA092103), and the CAS Interdisciplinary Innovation Team.
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| 课题组名称 | LNM微纳米流体力学
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| 论文分区 | 一类
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| 力学所作者排名 | False
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/59519
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| 专题 | 非线性力学国家重点实验室
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| 通讯作者 | Du, WB (reprint author), Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China. |
推荐引用方式
GB/T 7714 |
Xu P,Zheng X,Tao Y,et al. Cross-Interface Emulsification for Generating Size-Tunable Droplets[J]. ANALYTICAL CHEMISTRY,2016,88,6,:3171-3177.
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| APA |
Xu P,郑旭,Tao Y,Du WB,&Du, WB .(2016).Cross-Interface Emulsification for Generating Size-Tunable Droplets.ANALYTICAL CHEMISTRY,88(6),3171-3177.
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| MLA |
Xu P,et al."Cross-Interface Emulsification for Generating Size-Tunable Droplets".ANALYTICAL CHEMISTRY 88.6(2016):3171-3177.
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