IMECH-IR  > 非线性力学国家重点实验室
Cross-Interface Emulsification for Generating Size-Tunable Droplets
Xu P; Zheng X(郑旭); Tao Y; Du WB; Du, WB (reprint author), Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China.
Source PublicationANALYTICAL CHEMISTRY
2016
Volume88Issue:6Pages:3171-3177
ISSN0003-2700
AbstractWe report cross-interface emulsification (XiE), a pimple method for the generation of monodisperse droplets of controllable volumes from picoliter to nanoliter. A device is set up in which a fused-silica capillary Is vibrating across the surface of the continuous phase (mineral oil) in a reservoir, and the flow of the dispersed phase (aqueous solution) in the capillary is segmented into monodisperse droplets at the air/oil interface. We, find that the volume of droplets is mainly dominated by the flow rate and vibrating frequency and not significantly influenced by other factors,, such as the viscosity of the continuous phase and dispersed phase, the inner diameter of the capillary (20-100 mu m), Or the shape of the tip (tapered or flat). These features reflect high robustness, flexibility, and precision of XiE, for on-demand volume control of droplets. The droplets automatically assemble into planar monolayer droplet arrays (PMDA) in flat-bottomed microwells of 96-well plates, offering excellent convenience for imaging of droplets. As a representative application, we carry out digital loop-mediated isothermal amplification using PMDAs with multivolume droplets for the absolute quantification of nucleic acids. Our results demonstrate that XiE is simple and controllable for the production of monodisperse size-tunable droplets, and it offers opportunities for common laboratories, even without microfabrication facilities, to perform digital quantification, single cell analysis, and other biochemical assays with high throughput.
DOI10.1021/acs.analchem.5b04510
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000372391500028
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationThis work was supported by the program of China Ocean Mineral Resources R&D Association (DY125-15-R-02), the Strategic Priority Research Program of Chinese Academy of Sciences (XDB15040102), the National Natural Science Foundation of China (21205134, 31470221, 11202219, and 11572335), the National High Technology Research and Development Program of China (2012AA092103), and the CAS Interdisciplinary Innovation Team.
DepartmentLNM微纳米流体力学
Classification一类
RankingFalse
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/59519
Collection非线性力学国家重点实验室
Corresponding AuthorDu, WB (reprint author), Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China.
Recommended Citation
GB/T 7714
Xu P,Zheng X,Tao Y,et al. Cross-Interface Emulsification for Generating Size-Tunable Droplets[J]. ANALYTICAL CHEMISTRY,2016,88(6):3171-3177.
APA Xu P,郑旭,Tao Y,Du WB,&Du, WB .(2016).Cross-Interface Emulsification for Generating Size-Tunable Droplets.ANALYTICAL CHEMISTRY,88(6),3171-3177.
MLA Xu P,et al."Cross-Interface Emulsification for Generating Size-Tunable Droplets".ANALYTICAL CHEMISTRY 88.6(2016):3171-3177.
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