Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry

doi:10.1039/c6ay00089d

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	Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry
	Kang TF(康腾飞); Zhang KM; Yin CC; Niu Y(牛宇); Jin G(靳刚); Niu, Y; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
发表期刊	ANALYTICAL METHODS
	2016
卷号	8 期号:14 页码:2936-2940
ISSN	1759-9660
摘要	The interaction between the Homer EVH domain and RyR plays an important role in calcium signaling channels in the heart and the skeletal muscles. A biosensor based on imaging ellipsometry was used to analyze the binding affinity between the Homer EVH domain and RyR. The results confirm that the Homer EVH domain can recognize RyR specifically with high binding affinity of approximately 10(-8) M. This information will contribute to understanding of the role of the interaction between the Homer EVH domain and RyR in intracellular calcium channels.
DOI	10.1039/c6ay00089d
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000373440600010
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
WOS类目	Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
项目资助者	The authors acknowledge the financial support of the International Science & Technology Cooperation Program of China (2015DFG32390), the 7th Sino-Portugal Scientific and Technological Cooperation of 2013-2015, the National Basic Research Program of China (2015CB352100), and the National Natural Science Foundation of China (21305147) and (81472941).
课题组名称	NML纳米生物光学
论文分区	二类
力学所作者排名	True
引用统计
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/59521
专题	微重力重点实验室
通讯作者	Niu, Y; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Kang TF,Zhang KM,Yin CC,et al. Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry[J]. ANALYTICAL METHODS,2016,8,14,:2936-2940.
APA	康腾飞.,Zhang KM.,Yin CC.,牛宇.,靳刚.,...&Jin, G .(2016).Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry.ANALYTICAL METHODS,8(14),2936-2940.
MLA	康腾飞,et al."Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry".ANALYTICAL METHODS 8.14(2016):2936-2940.

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