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Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry
Huang CH; Yang QL; Song FY; Chen N; Yao B; Liao XL; Zhang ST; Chen YY; Jin G(靳刚); Huang, CH (reprint author), Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
Source PublicationINTEGRATED FERROELECTRICS
2016
Volume171Issue:1Pages:59-69
ISSN1058-4587
AbstractThis study describes the development of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate 19-9(CA19-9) multiplex immunoassay by integrated microfluidic device based on imaging ellipsometry (IMBIE). Different strategies for AFP, CEA and CA 19-9 simultaneous immunoassay on the same aldehyde-substrate were developed. Unifying the reaction steps of CA19-9 for antigen direct detection, CEA for secondary antibody (rabbit-antiCEA) addition, AFP for secondary (rabbit antiAFP) and an enhancer (goat IgG) application, and comprehensively considering the dynamic ranges and detection sensitivities, we combined these three markers into multiplex analysis in serial format.The sensitivities and dynamic ranges for CA19-9, CEA and AFP were 10.0U/mL, 1.0 ng/mL, 1.0 ng/mL and 2.0-100.0 U/mL, 0.1-64.0 ng/mL, 1.0-128.0 ng/mL, respectively. The intra-assay and inter-assay (coefficient variations) CVs of CA19-9, CEA and AFP were between 1.6-8.9% and 2.1-7.8%. The specificities tested for both cross-reaction and heterogeneous proteins of CA19-9, CEA and AFP were not more than 6.7%. Quantitative detection of 49 sera from gastric patients demonstrated that the accuracy for gastric cancer diagnosis was 83.4%. The total detection time is only 60min for an automated process. The proposed assay has the potential for practical application.
KeywordImaging Ellipsometry Immunoassay Afp Cea Ca19-9
DOI10.1080/10584587.2016.1171664
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000378406600009
WOS KeywordImaging ellipsometry ; immunoassay ; AFP ; CEA ; CA19-9
WOS Research AreaEngineering ; Physics
WOS SubjectEngineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter
Funding OrganizationThis work is supported by the cooperative project of academician workstation of Chongqing University of Science & Technology (CKYS2014Y5), Research Foundation of Chongqing University of Science & Technology (CK2014B4) and Chongqing pogram of Application Foundation and Advanced Technology(cstc2014jcyjA50032 cstc2014jcyjA10029).
DepartmentNML纳米生物光学
ClassificationQ4
RankingFalse
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/59532
Collection国家微重力实验室
Corresponding AuthorHuang, CH (reprint author), Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
Recommended Citation
GB/T 7714
Huang CH,Yang QL,Song FY,et al. Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2016,171(1):59-69
APA Huang CH.,Yang QL.,Song FY.,Chen N.,Yao B.,...&Jin, G .(2016).Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,171(1),59-69.
MLA Huang CH,et al."Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 171.1(2016):59-69.
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