Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry

doi:10.1080/10584587.2016.1171664

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	Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry
	Huang CH; Yang QL; Song FY; Chen N; Yao B; Liao XL; Zhang ST; Chen YY; Jin G(靳刚); Huang, CH (reprint author), Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
发表期刊	INTEGRATED FERROELECTRICS
	2016
卷号	171 期号:1 页码:59-69
ISSN	1058-4587
摘要	This study describes the development of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate 19-9(CA19-9) multiplex immunoassay by integrated microfluidic device based on imaging ellipsometry (IMBIE). Different strategies for AFP, CEA and CA 19-9 simultaneous immunoassay on the same aldehyde-substrate were developed. Unifying the reaction steps of CA19-9 for antigen direct detection, CEA for secondary antibody (rabbit-antiCEA) addition, AFP for secondary (rabbit antiAFP) and an enhancer (goat IgG) application, and comprehensively considering the dynamic ranges and detection sensitivities, we combined these three markers into multiplex analysis in serial format.The sensitivities and dynamic ranges for CA19-9, CEA and AFP were 10.0U/mL, 1.0 ng/mL, 1.0 ng/mL and 2.0-100.0 U/mL, 0.1-64.0 ng/mL, 1.0-128.0 ng/mL, respectively. The intra-assay and inter-assay (coefficient variations) CVs of CA19-9, CEA and AFP were between 1.6-8.9% and 2.1-7.8%. The specificities tested for both cross-reaction and heterogeneous proteins of CA19-9, CEA and AFP were not more than 6.7%. Quantitative detection of 49 sera from gastric patients demonstrated that the accuracy for gastric cancer diagnosis was 83.4%. The total detection time is only 60min for an automated process. The proposed assay has the potential for practical application.
关键词	Imaging Ellipsometry Immunoassay Afp Cea Ca19-9
DOI	10.1080/10584587.2016.1171664
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000378406600009
关键词[WOS]	Imaging ellipsometry ; immunoassay ; AFP ; CEA ; CA19-9
WOS研究方向	Engineering ; Physics
WOS类目	Engineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter
项目资助者	This work is supported by the cooperative project of academician workstation of Chongqing University of Science & Technology (CKYS2014Y5), Research Foundation of Chongqing University of Science & Technology (CK2014B4) and Chongqing pogram of Application Foundation and Advanced Technology(cstc2014jcyjA50032 cstc2014jcyjA10029).
课题组名称	NML纳米生物光学
论文分区	Q4
力学所作者排名	False
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/59532
专题	微重力重点实验室
通讯作者	Huang, CH (reprint author), Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
推荐引用方式 GB/T 7714	Huang CH,Yang QL,Song FY,et al. Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2016,171,1,:59-69.
APA	Huang CH.,Yang QL.,Song FY.,Chen N.,Yao B.,...&Jin, G .(2016).Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,171(1),59-69.
MLA	Huang CH,et al."Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 171.1(2016):59-69.

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