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The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association
Wei JC(韦佳辰); Dobnikar J; Curk T; Song F(宋凡); Song, F (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China.
Source PublicationPLOS ONE
2016
Volume11Issue:3Pages:e0151159
ISSN1932-6203
AbstractIn living systems proteins are typically found in crowded environments where their effective interactions strongly depend on the surrounding medium. Yet, their association and dissociation needs to be robustly controlled in order to enable biological function. Uncontrolled protein aggregation often causes disease. For instance, cataract is caused by the clustering of lens proteins, i.e., crystallins, resulting in enhanced light scattering and impaired vision or blindness. To investigate the molecular origins of cataract formation and to design efficient treatments, a better understanding of crystallin association in macromolecular crowded environment is needed. Here we present a theoretical study of simple coarse grained colloidal models to characterize the general features of how the association equilibrium of proteins depends on the magnitude of intermolecular attraction. By comparing the analytic results to the available experimental data on the osmotic pressure in crystallin solutions, we identify the effective parameters regimes applicable to crystallins. Moreover, the combination of two models allows us to predict that the number of binding sites on crystallin is small, i.e. one to three per protein, which is different from previous estimates. We further observe that the crowding factor is sensitive to the size asymmetry between the reactants and crowding agents, the shape of the protein clusters, and to small variations of intermolecular attraction. Our work may provide general guidelines on how to steer the protein interactions in order to control their association.
DOI10.1371/journal.pone.0151159
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000371991300090
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
Funding OrganizationThis work was supported by the National Natural Science Foundation of China (Grant No. 11232013 and No. 11472285) and the Fundamental Research Funds for the Central Universities of P. R. China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Natural Science Foundations of China ( Grants No. 11232013 and No. 11472285) and the Fundamental Research Funds for the Central Universities of P. R. China under the project buctrc201422.
DepartmentLNM生物材料微结构和力学性能
Classification二类
RankingTrue
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/59570
Collection非线性力学国家重点实验室
Corresponding AuthorSong, F (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Wei JC,Dobnikar J,Curk T,et al. The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association[J]. PLOS ONE,2016,11(3):e0151159.
APA Wei JC,Dobnikar J,Curk T,Song F,&Song, F .(2016).The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association.PLOS ONE,11(3),e0151159.
MLA Wei JC,et al."The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association".PLOS ONE 11.3(2016):e0151159.
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