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The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism
Zou JJ; Zheng ZY(郑中玉); Xue S; Li HH(李瀚海); Wang YR(王育人); Le J; Le, J (reprint author), Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China.
Source PublicationJOURNAL OF EXPERIMENTAL BOTANY
2016-10-01
Volume67Issue:18Pages:5325-5337
ISSN0022-0957
Abstract

Arabidopsis actin-related protein ARP3 plays a role in amyloplast sedimentation and polar auxin redistribution during root gravitropism.Gravitropism is vital for shaping directional plant growth in response to the forces of gravity. Signals perceived in the gravity-sensing cells can be converted into biochemical signals and transmitted. Sedimentation of amyloplasts in the columella cells triggers asymmetric auxin redistribution in root tips, leading to downward root growth. The actin cytoskeleton is thought to play an important role in root gravitropism, although the molecular mechanism has not been resolved. DISTORTED1 (DIS1) encodes the ARP3 subunit of the Arabidopsis Actin-Related Protein 2/3 (ARP2/3) complex, and the ARP3/DIS1 mutant dis1-1 showed delayed root curvature after gravity stimulation. Microrheological analysis revealed that the high apparent viscosity within dis1-1 central columella cells is closely associated with abnormal movement trajectories of amyloplasts. Analysis using a sensitive auxin input reporter DII-VENUS showed that asymmetric auxin redistribution was reduced in the root tips of dis1-1, and the actin-disrupting drug Latrunculin B increased the asymmetric auxin redistribution. An uptake assay using the membrane-selective dye FM4-64 indicated that endocytosis was decelerated in dis1-1 root epidermal cells. Treatment and wash-out with Brefeldin A, which inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus, showed that cycling of the auxin-transporter PIN-FORMED (PIN) proteins to the plasma membrane was also suppressed in dis1-1 roots. The results reveal that ARP3/DIS1 acts in root gravitropism by affecting amyloplast sedimentation and PIN-mediated polar auxin transport through regulation of PIN protein trafficking.

KeywordAmyloplast Arabidopsis Arp2/3 Auxin Gravitropism Pins
DOI10.1093/jxb/erw294
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000386067000006
WOS KeywordARG1 ALTERED-RESPONSE ; ARP2/3 COMPLEX ; COLUMELLA CELLS ; EFFLUX CARRIER ; F-ACTIN ; PLANT GRAVITROPISM ; CYTOSKELETON ; DYNAMICS ; PIN2 ; THALIANA
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
Funding OrganizationNational Basic Research Program of China(2011CB710902 ; Strategic Pioneer Program on Space Science, Chinese Academy of Sciences(XDA04079100) ; 2011CB710901)
DepartmentNML空间材料物理力学
Classification一类
RankingFalse
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/59699
Collection国家微重力实验室
Corresponding AuthorLe, J (reprint author), Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China.
Recommended Citation
GB/T 7714
Zou JJ,Zheng ZY,Xue S,et al. The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(18):5325-5337.
APA Zou JJ.,Zheng ZY.,Xue S.,Li HH.,Wang YR.,...&Le, J .(2016).The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism.JOURNAL OF EXPERIMENTAL BOTANY,67(18),5325-5337.
MLA Zou JJ,et al."The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism".JOURNAL OF EXPERIMENTAL BOTANY 67.18(2016):5325-5337.
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