IMECH-IR  > 国家微重力实验室
Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins
Jiang MM; Qiu JH; Zhang LL; Lu DY; Long M(龙勉); Che, L; Luo XD; Luo, XD (reprint author), Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China.; Chen, L (reprint author), Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China.
Source PublicationEXPERIMENTAL AND THERAPEUTIC MEDICINE
2016
Volume12Issue:3Pages:1542-1550
ISSN1792-0981
AbstractWound healing is a complicated but highly organized process in which cell migration and proliferation are actively involved. However, the process by which mechanical stretch regulates the proliferation and migration of human skin fibroblasts (HFs) and keratinocytes is poorly understood. Using a house built mechanical stretch device, we examined the HFs extracellular matrix (ECM) components changes under non-stretch, static stretch or cyclic stretch conditions. We further investigated the changes in ECM component protein expression levels in keratinocytes and analyzed the effects of individual ECM component on keratinocyte proliferation and migration. Particularly, the roles of calcium/calmodulin-dependent serine protein kinase (CASK) in the HF proliferation under cyclic stretch were investigated. Cyclic stretch suppressed HF proliferation compared with HFs without stretch or with static stretch. Cyclic stretch also led to a significant reduction in the levels of collagen I and a marked increase of fibronectin in HFs ECM. By contrast, collagen I levels increased and fibronectin levels decreased in response to non-stretch and static stretch conditions. After cyclic stretch, the proliferation of keratinocytes was inhibited by the cyclic stretch-induced ECM in HFs. The inoculation of keratinocytes with single ECM component suggested that collagen I was more capable of inducing cell proliferation than fibronectin, while it had less impact on cell migration compared with fibronectin. Furthermore, cyclic stretch induced by proliferation inhibition was associated with altered integrin 1-CASK signal pathway. The present results demonstrated the existence of HF-ECM-keratinocyte cross-talk' in cutaneous tissues. Thus, the integrin 1-CASK signal pathway in HFs may be involved in the outside-in signal transduction of extracellular stretch and the altered ECM component expression.
KeywordHuman Skin Fibroblast Keratinocyte Mechanical Stretch Extracellular Matrix Integrin Calcium Calmodulin-dependent Serine Protein Kinase
DOI10.3892/etm.2016.3497
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000382446000047
WOS Keywordhuman skin fibroblast ; keratinocyte ; mechanical stretch ; extracellular matrix ; integrin ; calcium ; calmodulin-dependent serine protein kinase
WOS Research AreaResearch & Experimental Medicine
WOS SubjectMedicine, Research & Experimental
Funding OrganizationThis study was supported by grants from the National High Technology Research and Development Program of China (grant no. 2011CB710904) and the National Natural Science Foundation of China (grant no. 81372813).
DepartmentNML分子-细胞生物力学与空间生命科学
ClassificationQ4
RankingFalse
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/59743
Collection国家微重力实验室
Corresponding AuthorLuo, XD (reprint author), Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China.; Chen, L (reprint author), Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China.
Recommended Citation
GB/T 7714
Jiang MM,Qiu JH,Zhang LL,et al. Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2016,12(3):1542-1550.
APA Jiang MM.,Qiu JH.,Zhang LL.,Lu DY.,龙勉.,...&Chen, L .(2016).Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins.EXPERIMENTAL AND THERAPEUTIC MEDICINE,12(3),1542-1550.
MLA Jiang MM,et al."Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins".EXPERIMENTAL AND THERAPEUTIC MEDICINE 12.3(2016):1542-1550.
Files in This Item: Download All
File Name/Size DocType Version Access License
a2016-054.pdf(1118KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jiang MM]'s Articles
[Qiu JH]'s Articles
[Zhang LL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jiang MM]'s Articles
[Qiu JH]'s Articles
[Zhang LL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jiang MM]'s Articles
[Qiu JH]'s Articles
[Zhang LL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: a2016-054.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.