Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins

doi:10.3892/etm.2016.3497

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	Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins
	Jiang MM; Qiu JH; Zhang LL; Lu DY; Long M(龙勉); Che, L; Luo XD; Luo, XD (reprint author), Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China.; Chen, L (reprint author), Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China.
发表期刊	EXPERIMENTAL AND THERAPEUTIC MEDICINE
	2016
卷号	12 期号:3 页码:1542-1550
ISSN	1792-0981
摘要	Wound healing is a complicated but highly organized process in which cell migration and proliferation are actively involved. However, the process by which mechanical stretch regulates the proliferation and migration of human skin fibroblasts (HFs) and keratinocytes is poorly understood. Using a house built mechanical stretch device, we examined the HFs extracellular matrix (ECM) components changes under non-stretch, static stretch or cyclic stretch conditions. We further investigated the changes in ECM component protein expression levels in keratinocytes and analyzed the effects of individual ECM component on keratinocyte proliferation and migration. Particularly, the roles of calcium/calmodulin-dependent serine protein kinase (CASK) in the HF proliferation under cyclic stretch were investigated. Cyclic stretch suppressed HF proliferation compared with HFs without stretch or with static stretch. Cyclic stretch also led to a significant reduction in the levels of collagen I and a marked increase of fibronectin in HFs ECM. By contrast, collagen I levels increased and fibronectin levels decreased in response to non-stretch and static stretch conditions. After cyclic stretch, the proliferation of keratinocytes was inhibited by the cyclic stretch-induced ECM in HFs. The inoculation of keratinocytes with single ECM component suggested that collagen I was more capable of inducing cell proliferation than fibronectin, while it had less impact on cell migration compared with fibronectin. Furthermore, cyclic stretch induced by proliferation inhibition was associated with altered integrin 1-CASK signal pathway. The present results demonstrated the existence of HF-ECM-keratinocyte cross-talk' in cutaneous tissues. Thus, the integrin 1-CASK signal pathway in HFs may be involved in the outside-in signal transduction of extracellular stretch and the altered ECM component expression.
关键词	Human Skin Fibroblast Keratinocyte Mechanical Stretch Extracellular Matrix Integrin Calcium Calmodulin-dependent Serine Protein Kinase
DOI	10.3892/etm.2016.3497
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000382446000047
关键词[WOS]	human skin fibroblast ; keratinocyte ; mechanical stretch ; extracellular matrix ; integrin ; calcium ; calmodulin-dependent serine protein kinase
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
项目资助者	This study was supported by grants from the National High Technology Research and Development Program of China (grant no. 2011CB710904) and the National Natural Science Foundation of China (grant no. 81372813).
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	Q4
力学所作者排名	False
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/59743
专题	微重力重点实验室
通讯作者	Luo, XD (reprint author), Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China.; Chen, L (reprint author), Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China.
推荐引用方式 GB/T 7714	Jiang MM,Qiu JH,Zhang LL,et al. Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2016,12,3,:1542-1550.
APA	Jiang MM.,Qiu JH.,Zhang LL.,Lu DY.,龙勉.,...&Chen, L .(2016).Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins.EXPERIMENTAL AND THERAPEUTIC MEDICINE,12(3),1542-1550.
MLA	Jiang MM,et al."Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins".EXPERIMENTAL AND THERAPEUTIC MEDICINE 12.3(2016):1542-1550.

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