Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios | |
Zhang L; Sun JS; Wang YL; Wang JC; Shi XH; Hu GQ(胡国庆); Sun, JS (reprint author), Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China. | |
发表期刊 | ANALYTICAL CHEMISTRY |
2016 | |
卷号 | 88期号:14页码:7344-7351 |
ISSN | 0003-2700 |
摘要 | We report a nonspecific organelle-targeting strategy through one-step microfluidic fabrication and screening of a library of surface charge- and lipid components/ratios-varied lipid shell polymer core nanoparticles. Different from the common strategy relying on the use of organelle-targeted moieties conjugated onto the surface of nanoparticles, here, we program the distribution of hybrid nanoparticles in lysosomes or mitochondria by tuning the lipid components/ratios in shell. Hybrid nanoparticles with 60% 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and 20% 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) can intracellularly target mitochondria in both in vitro and in vivo models. While replacing DOPE with the same amount of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), the nanoparticles do not show mitochondrial targeting, indicating an incremental effect of cationic and fusogenic lipids on lysosomal escape which is further studied by molecular dynamics simulations. This work unveils the lipid-regulated subcellular distribution of hybrid nanoparticles in which target moieties and complex synthetic steps are avoided. |
DOI | 10.1021/acs.analchem.6b01749 |
URL | 查看原文 |
收录类别 | SCI ; EI |
语种 | 英语 |
WOS记录号 | WOS:000380296800054 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
项目资助者 | We acknowledge financial support from MOST (2013AA032204), NSFC (21475028, 11422215, 11272327, and 11023001), and Youth Innovation Promotion Association CAS. |
课题组名称 | LNM微纳米流体力学 |
论文分区 | 一类 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://dspace.imech.ac.cn/handle/311007/59761 |
专题 | 非线性力学国家重点实验室 |
通讯作者 | Sun, JS (reprint author), Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China. |
推荐引用方式 GB/T 7714 | Zhang L,Sun JS,Wang YL,et al. Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios[J]. ANALYTICAL CHEMISTRY,2016,88,14,:7344-7351. |
APA | Zhang L.,Sun JS.,Wang YL.,Wang JC.,Shi XH.,...&Sun, JS .(2016).Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios.ANALYTICAL CHEMISTRY,88(14),7344-7351. |
MLA | Zhang L,et al."Nonspecific Organelle-Targeting Strategy with Core-Shell Nanoparticles of Varied Lipid Components/Ratios".ANALYTICAL CHEMISTRY 88.14(2016):7344-7351. |
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