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beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model
Lv DY(吕东媛); Li Z; Gao YX(高宇欣); Luo CH; Zhang F; Zheng L; Wang JW; Sun SJ(孙树津); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.
Source PublicationBIOMEDICAL ENGINEERING ONLINE
2016
Volume15Pages:130
ISSN1475-925X
Abstract

Background: Keratinocyte (KC) migration in re-epithelization is crucial in repairing injured skin. But the mechanisms of how mechanical stimuli regulate the migration of keratinocytes have been poorly understood. Methods: Human immortalized keratinocyte HaCaT cells were co-cultured with skin fibroblasts on PDMS membranes and transferred to the static stretch device developed in-house for additional 6 day culture under mechanical stretch to mimic surface tension in skin. To detect the expression of proteins on different position at different time points and the effect of beta 1 integrin mechanotransduction on HaCaT migration, Immunofluorescence, Reverse transcription-polymerase chain reaction, Flow cytometry, Western blotting assays were applied. Results: Mechanical receptor of beta 1 integrin that recognizes its ligand of collagen I was found to be strongly associated with migration of HaCaT cells since the knockdown of beta 1 integrin via RNA silence eliminated the key protein expression dynamically. Here the expression of vinculin was lower but that of Cdc42 was higher for the cells at outward edge than those at inward edge, respectively, supporting that the migration capability of keratinocytes is inversely correlated with the formation of focal adhesion complexes but positively related to the lamellipodia formation. This asymmetric expression feature was further confirmed by high or low expression of PI3K for outward-or inward-migrating cells. And ERK1/ 2 phosphorylation was up-regulated by mechanical stretch. Conclusion: We reported here, a novel mechanotransduction signaling pathways were beta 1 integrin-dependent pattern of keratinocytes migration under static stretch in an in vitro co-culture model. These results provided an insight into underlying molecular mechanisms of keratinocyte migration under mechanical stimuli.

DOI10.1186/s12938-016-0263-1
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000392424700006
WOS KeywordKeratinocyte ; Mechanical stretch ; Fibroblast ; beta 1 integrin ; Mechanotransduction
WOS Research AreaEngineering
WOS SubjectEngineering, Biomedical
Funding OrganizationPublication of this article was funded by National Natural Science Foundation of China Grants 31110103918 and 31470907.
DepartmentNML分子-细胞生物力学与空间生命科学
ClassificationQ3
RankingTrue
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/60090
Collection国家微重力实验室
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Lv DY,Li Z,Gao YX,et al. beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model[J]. BIOMEDICAL ENGINEERING ONLINE,2016,15:130.
APA Lv DY.,Li Z.,Gao YX.,Luo CH.,Zhang F.,...&Long, M .(2016).beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model.BIOMEDICAL ENGINEERING ONLINE,15,130.
MLA Lv DY,et al."beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model".BIOMEDICAL ENGINEERING ONLINE 15(2016):130.
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