IMECH-IR  > 力学所知识产出(1956-2008)
Quantifying rupture force and bond lifetime of P-selectin-PSGL-1 interactions at lower forces
Zhang Y(章燕); Sun GY(孙淦云); Huo B(霍波); Long M(龙勉)
Source PublicationAdvances in Heterogeneous Material Mechanics 2008 - Proceedings of the 2nd International Conference on Heterogeneous Material Mechanics, ICHMM 2008
Conference NameAdvances in Heterogeneous Material Mechanics 2008 - 2nd International Conference on Heterogeneous Material Mechanics, ICHMM 2008
Conference DateJune 3, 2008 - June 8, 2008
Conference PlaceHuangshan, China
AbstractCell adhesions mediated by receptor-ligand interactions are crucial to many biological processes such as inflammatory cascade, tumor metastasis, and thrombosis formation. Rupture force of receptor-ligand bond is enhanced with logarithm of loading rate (= spring constant × retracting velocity). Bond lifetime is found to be prolonged (so-called catch bond) or shortened (so-called slip bond) with applied forces and presents catch-slip bond transition, when atomic force microscopy and flow chamber assays are used to monitor bond rupture at relatively higher force rang (> 10 pN). The mechanism how bond rupture is regulated at lower forces (-3 pN/nm was used to trap two silicon beads coupled by P-selectin and PSGL-1 molecules via their respective capturing antibodies (Fig. 1). Bond rupture forces and lifetimes were measured at systematically-varied trap spring constants and retracting velocities. Most probable rupture forces were enhanced when higher spring constants were used at a given retracting velocity, but remained same when higher retracting velocities were applied at a pre-set spring constant (except at the highest constant of 0.047 pN/nm), suggesting that the spring constant of optical trap plays an more important role in regulating forced bond dissociation (Fig. 2). Bond lifetime exhibited a slip-catch bond transition when applied forces varied from 0.5 to 15 pN, validating the hypothesis that another slip-catch bond transition phase appears when lower external forces are applied. It was also found that, at a given applied force, bond lifetime varied when high spring constant with low retracting velocity or low spring constant with high retracting velocity, was used. These findings further the understanding of forced dissociation of receptor-ligand bonds at lower applied forces, and provide a new insight into manipulating the cell adhesions in physiological flow.
KeywordApplied Forces Atomic Forces Biological Processes Bond Dissociations Bond Lifetimes Bond Ruptures External Forces Flow Chambers Glycoprotein Ligands Life-times Ligand Interactions Loading Rates Optical Traps Physiological Flows Rupture Forces Selectin Spring Constants Thrombosis Formations To Many Tumor Metastases
WOS IDWOS:000257167700285
Indexed ByCPCI-S ; EI
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Document Type会议论文
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GB/T 7714
Zhang Y,Sun GY,Huo B,et al. Quantifying rupture force and bond lifetime of P-selectin-PSGL-1 interactions at lower forces[C],2008.
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