|Nanostructured interfaces with site-specific bioreceptors for immunosensing|
|Paiva TO; Almeida I; Marques JT; Liu W; Niu Y(牛宇); Jin G(靳刚); Viana AS; Viana, AS (reprint author), Univ Lisbon, Ctr Quim & Bioquim, Fac Ciencias, Edificio C8, P-1749016 Lisbon, Portugal.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, Beijing 100190, Peoples R China.
|Source Publication||APPLIED SURFACE SCIENCE
|Abstract||In this work we propose a simple and effective approach to build nanostructured immunosensor platforms. The one-step strategy relies on i) the in situ formation of dithiocarbamates from the reaction between carbon disulfide and amine groups present on protein A ii) their attachment to gold nanopartides (AuNPs) and iii) the linkage of the modified AuNPs to the electrode surface which depends on the strong interaction between gold substrates and sulfur moieties. AuNPs and protein A are used to increase the surface coverage of Immunoglobulin G (IgG) and promote the oriented immobilization of the antibodies on the immunosensing interface. The modified gold surfaces with biomolecules were thoroughly characterized by a combination of techniques: UV-vis spectrophotometry conventional ellipsometry and atomic force microscopy. The immunosensor performance was assessed in real-time by surface plasmon resonance and by the highly sensitive total internal reflection imaging ellipsometry through the specific biorecognition between anti-IgG and the immobilized IgG molecules. We demonstrate that the presence of AuNPs improves the sensitivity of the anti-IgG specific detection whereas the presence of co-adsorbed CS2 is responsible for blocking the undesired protein nonspecific adsorption to the gold substrate. Overall we report a simple and innovative one-step method to chemically modify gold surfaces with protein A and AuNPs able to specifically detect antigen antibody interactions with capability of preventing protein nonspecific adsorption. (C) 2017 Elsevier B.V. All rights reserved.|
|Keyword||Gold Surface Modification
Protein a Nanobioconjugates
In Situ Dithiocarbamate Formation
Inhibition Of Nonspecific Adsorption
Antibody Oriented Immobilization
Total Internal Reflection Imaging
|WOS Keyword||SELF-ASSEMBLED MONOLAYERS
; SURFACE-PLASMON RESONANCE
; INTERNAL-REFLECTION ELLIPSOMETRY
; NANOPARTICLE THIN-FILMS
; GOLD NANOPARTICLES
; ORIENTED IMMOBILIZATION
; IMAGING ELLIPSOMETRY
; SERUM SAMPLES
|WOS Research Area||Chemistry
; Materials Science
|WOS Subject||Chemistry, Physical
; Materials Science, Coatings & Films
; Physics, Applied
; Physics, Condensed Matter
|Funding Organization||Fundacao para a Ciencia e a Tecnologia (FCT) through: 7th Sino-Portugal Scientific and Technological Cooperation(PEst 2015-2020-UID/Multi/00612/2013
; International Science & Technology Cooperation Program of China(2015DFG32390)
; National Basic Research Program of China(2015CB3521009)
; National Natural Science Foundation of China(21305147
|Corresponding Author||Viana, AS (reprint author), Univ Lisbon, Ctr Quim & Bioquim, Fac Ciencias, Edificio C8, P-1749016 Lisbon, Portugal.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, Beijing 100190, Peoples R China.|
Paiva TO,Almeida I,Marques JT,et al. Nanostructured interfaces with site-specific bioreceptors for immunosensing[J]. APPLIED SURFACE SCIENCE,2017,412:455-463.
Paiva TO.,Almeida I.,Marques JT.,Liu W.,牛宇.,...&Jin, G .(2017).Nanostructured interfaces with site-specific bioreceptors for immunosensing.APPLIED SURFACE SCIENCE,412,455-463.
Paiva TO,et al."Nanostructured interfaces with site-specific bioreceptors for immunosensing".APPLIED SURFACE SCIENCE 412(2017):455-463.