Nanostructured interfaces with site-specific bioreceptors for immunosensing | |
Paiva TO; Almeida I; Marques JT; Liu W(刘巍); Niu Y(牛宇); Jin G(靳刚); Viana AS; Viana, AS (reprint author), Univ Lisbon, Ctr Quim & Bioquim, Fac Ciencias, Edificio C8, P-1749016 Lisbon, Portugal.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, Beijing 100190, Peoples R China. | |
发表期刊 | APPLIED SURFACE SCIENCE |
2017-08-01 | |
卷号 | 412页码:455-463 |
ISSN | 0169-4332 |
摘要 | In this work we propose a simple and effective approach to build nanostructured immunosensor platforms. The one-step strategy relies on i) the in situ formation of dithiocarbamates from the reaction between carbon disulfide and amine groups present on protein A ii) their attachment to gold nanopartides (AuNPs) and iii) the linkage of the modified AuNPs to the electrode surface which depends on the strong interaction between gold substrates and sulfur moieties. AuNPs and protein A are used to increase the surface coverage of Immunoglobulin G (IgG) and promote the oriented immobilization of the antibodies on the immunosensing interface. The modified gold surfaces with biomolecules were thoroughly characterized by a combination of techniques: UV-vis spectrophotometry conventional ellipsometry and atomic force microscopy. The immunosensor performance was assessed in real-time by surface plasmon resonance and by the highly sensitive total internal reflection imaging ellipsometry through the specific biorecognition between anti-IgG and the immobilized IgG molecules. We demonstrate that the presence of AuNPs improves the sensitivity of the anti-IgG specific detection whereas the presence of co-adsorbed CS2 is responsible for blocking the undesired protein nonspecific adsorption to the gold substrate. Overall we report a simple and innovative one-step method to chemically modify gold surfaces with protein A and AuNPs able to specifically detect antigen antibody interactions with capability of preventing protein nonspecific adsorption. (C) 2017 Elsevier B.V. All rights reserved. |
关键词 | Gold Surface Modification Protein a Nanobioconjugates In Situ Dithiocarbamate Formation Inhibition Of Nonspecific Adsorption Antibody Oriented Immobilization Total Internal Reflection Imaging Ellipsometry |
DOI | 10.1016/j.apsusc.2017.03.180 |
收录类别 | SCI ; EI |
语种 | 英语 |
WOS记录号 | WOS:000401392900051 |
关键词[WOS] | SELF-ASSEMBLED MONOLAYERS ; SURFACE-PLASMON RESONANCE ; INTERNAL-REFLECTION ELLIPSOMETRY ; NANOPARTICLE THIN-FILMS ; GOLD NANOPARTICLES ; ORIENTED IMMOBILIZATION ; IMAGING ELLIPSOMETRY ; CARBON-DISULFIDE ; SERUM SAMPLES ; PROTEIN |
WOS研究方向 | Chemistry ; Materials Science ; Physics |
WOS类目 | Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
项目资助者 | Fundacao para a Ciencia e a Tecnologia (FCT) through: 7th Sino-Portugal Scientific and Technological Cooperation(PEst 2015-2020-UID/Multi/00612/2013 ; International Science & Technology Cooperation Program of China(2015DFG32390) ; National Basic Research Program of China(2015CB3521009) ; National Natural Science Foundation of China(21305147 ; PTDC/CTM-NAN/0994/2014 ; 81472941) ; SFRH/BD/70673/2010 ; IF/00808/2013) |
论文分区 | 一类 |
力学所作者排名 | 1 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://dspace.imech.ac.cn/handle/311007/60526 |
专题 | 微重力重点实验室 |
通讯作者 | Viana, AS (reprint author), Univ Lisbon, Ctr Quim & Bioquim, Fac Ciencias, Edificio C8, P-1749016 Lisbon, Portugal.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, Beijing 100190, Peoples R China. |
推荐引用方式 GB/T 7714 | Paiva TO,Almeida I,Marques JT,et al. Nanostructured interfaces with site-specific bioreceptors for immunosensing[J]. APPLIED SURFACE SCIENCE,2017,412:455-463. |
APA | Paiva TO.,Almeida I.,Marques JT.,Liu W.,Niu Y.,...&Jin, G .(2017).Nanostructured interfaces with site-specific bioreceptors for immunosensing.APPLIED SURFACE SCIENCE,412,455-463. |
MLA | Paiva TO,et al."Nanostructured interfaces with site-specific bioreceptors for immunosensing".APPLIED SURFACE SCIENCE 412(2017):455-463. |
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