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Title:
Nanostructured interfaces with site-specific bioreceptors for immunosensing
Author: Paiva TO; Almeida I; Marques JT; Liu W; Niu Y(牛宇); Jin G(靳刚); Viana AS
Source: APPLIED SURFACE SCIENCE
Issued Date: 2017-08-01
Volume: 412, Pages:455-463
Abstract: In this work we propose a simple and effective approach to build nanostructured immunosensor platforms. The one-step strategy relies on i) the in situ formation of dithiocarbamates from the reaction between carbon disulfide and amine groups present on protein A ii) their attachment to gold nanopartides (AuNPs) and iii) the linkage of the modified AuNPs to the electrode surface which depends on the strong interaction between gold substrates and sulfur moieties. AuNPs and protein A are used to increase the surface coverage of Immunoglobulin G (IgG) and promote the oriented immobilization of the antibodies on the immunosensing interface. The modified gold surfaces with biomolecules were thoroughly characterized by a combination of techniques: UV-vis spectrophotometry conventional ellipsometry and atomic force microscopy. The immunosensor performance was assessed in real-time by surface plasmon resonance and by the highly sensitive total internal reflection imaging ellipsometry through the specific biorecognition between anti-IgG and the immobilized IgG molecules. We demonstrate that the presence of AuNPs improves the sensitivity of the anti-IgG specific detection whereas the presence of co-adsorbed CS2 is responsible for blocking the undesired protein nonspecific adsorption to the gold substrate. Overall we report a simple and innovative one-step method to chemically modify gold surfaces with protein A and AuNPs able to specifically detect antigen antibody interactions with capability of preventing protein nonspecific adsorption. (C) 2017 Elsevier B.V. All rights reserved.
Keyword: Gold surface modification ; Protein A nanobioconjugates ; In situ dithiocarbamate formation ; Inhibition of nonspecific adsorption ; Antibody oriented immobilization ; Total internal reflection imaging ; ellipsometry
Language: 英语
Indexed Type: SCI ; EI
DOI: 10.1016/j.apsusc.2017.03.180
DOC Type: Article
WOS Subject: Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
WOS Subject Extended: Chemistry ; Materials Science ; Physics
WOS Keyword Plus: SELF-ASSEMBLED MONOLAYERS ; SURFACE-PLASMON RESONANCE ; INTERNAL-REFLECTION ELLIPSOMETRY ; NANOPARTICLE THIN-FILMS ; GOLD NANOPARTICLES ; ORIENTED IMMOBILIZATION ; IMAGING ELLIPSOMETRY ; CARBON-DISULFIDE ; SERUM SAMPLES ; PROTEIN
WOS ID: WOS:000401392900051
ISSN: 0169-4332
Funder: Fundacao para a Ciencia e a Tecnologia (FCT) through: 7th Sino-Portugal Scientific and Technological Cooperation(PEst 2015-2020-UID/Multi/00612/2013 ; International Science & Technology Cooperation Program of China(2015DFG32390) ; National Basic Research Program of China(2015CB3521009) ; National Natural Science Foundation of China(21305147 ; PTDC/CTM-NAN/0994/2014 ; 81472941) ; SFRH/BD/70673/2010 ; IF/00808/2013)
Department: NML纳米生物光学
First Institution (Yes or Not): false
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Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/60526
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
Paiva TO,Almeida I,Marques JT,et al. Nanostructured interfaces with site-specific bioreceptors for immunosensing[J]. APPLIED SURFACE SCIENCE,2017-08-01,412:455-463.
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