| Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow |
| Yang H(杨浩); Li N(李宁) ; Du Y(杜宇); Tong CF(佟春芳); Lv SQ(吕守芹); Hu JR; Zhang Y(章燕); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
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| 发表期刊 | EXPERIMENTAL CELL RESEARCH
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| 2017-02-01
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| 卷号 | 351期号:1页码:91-99 |
| ISSN | 0014-4827
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| 摘要 | Neutrophil (polymorphonuclear leukocyte PMN) recruitment in the liver sinusoid takes place in almost all liver diseases and contributes to pathogen clearance or tissue damage. While PMN rolling unlikely appears in liver sinusoids and Mac-1 or CD44 is assumed to play respective roles during in vivo local or systematic inflammatory stimulation the regulating mechanisms of PMN adhesion and crawling dynamics are still unclear from those in vivo studies. Here we developed a two-dimensional in vitro sinusoidal model with primary liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs) to investigate TNF-alpha-induced PMN recruitment under shear flow. Our data demonstrated that LFA-1 dominates the static or shear resistant adhesion of PMNs while Mac-1 decelerates PMN crawling on LSEC monolayer. Any one of LFA-1 Mac-1 and CD44 molecules is not able to work effectively for mediating PMN transmigration across LSEC monolayer. The presence of KCs only affects the randomness of PMN crawling. These findings further the understandings of PMN recruitment under shear flow in liver sinusoids. |
| 关键词 | Neutrophils
Liver Sinusoids
Shear Flow
Adhesive Molecules
Kupffer Cells
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| DOI | 10.1016/j.yexcr.2017.01.002
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| 收录类别 | SCI
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| 语种 | 英语
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| WOS记录号 | WOS:000394196700011
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| 关键词[WOS] | BLOOD-BRAIN-BARRIER
; INFLAMED LIVER
; IN-VITRO
; HEPATIC MICROCIRCULATION
; MOLECULE-1 EXPRESSION
; LEUKOCYTE RECRUITMENT
; EXTRACELLULAR-MATRIX
; ISCHEMIA-REPERFUSION
; STERILE INFLAMMATION
; INJURY
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| WOS研究方向 | Oncology
; Cell Biology
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| WOS类目 | Oncology
; Cell Biology
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| 项目资助者 | National Natural Science Foundation of China(31230027
; Frontier Science Key Project of Chinese Academy of Sciences(QYZDJ-SSW-JSC018
; Strategic Priority Research Program of Chinese Academy of Sciences(QYZDJ-SSW-JSC018
; 91642203
; XDB22040101)
; XDB22040101)
; 31661143044
; 31110103918
; 31300776)
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| 课题组名称 | NML分子-细胞生物力学与空间生命科学
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| 论文分区 | 二类
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| 力学所作者排名 | 1
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/60576
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| 专题 | 微重力重点实验室
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| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China. |
推荐引用方式
GB/T 7714 |
Yang H,Li N,Du Y,et al. Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow[J]. EXPERIMENTAL CELL RESEARCH,2017,351,1,:91-99.
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| APA |
杨浩.,李宁.,杜宇.,佟春芳.,吕守芹.,...&Long, M .(2017).Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow.EXPERIMENTAL CELL RESEARCH,351(1),91-99.
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| MLA |
杨浩,et al."Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow".EXPERIMENTAL CELL RESEARCH 351.1(2017):91-99.
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