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Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow
Yang H(杨浩); Li N(李宁); Du Y(杜宇); Tong CF(佟春芳); Lv SQ(吕守芹); Hu JR; Zhang Y(章燕); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
Source PublicationEXPERIMENTAL CELL RESEARCH
2017-02-01
Volume351Issue:1Pages:91-99
ISSN0014-4827
AbstractNeutrophil (polymorphonuclear leukocyte PMN) recruitment in the liver sinusoid takes place in almost all liver diseases and contributes to pathogen clearance or tissue damage. While PMN rolling unlikely appears in liver sinusoids and Mac-1 or CD44 is assumed to play respective roles during in vivo local or systematic inflammatory stimulation the regulating mechanisms of PMN adhesion and crawling dynamics are still unclear from those in vivo studies. Here we developed a two-dimensional in vitro sinusoidal model with primary liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs) to investigate TNF-alpha-induced PMN recruitment under shear flow. Our data demonstrated that LFA-1 dominates the static or shear resistant adhesion of PMNs while Mac-1 decelerates PMN crawling on LSEC monolayer. Any one of LFA-1 Mac-1 and CD44 molecules is not able to work effectively for mediating PMN transmigration across LSEC monolayer. The presence of KCs only affects the randomness of PMN crawling. These findings further the understandings of PMN recruitment under shear flow in liver sinusoids.
KeywordNeutrophils Liver Sinusoids Shear Flow Adhesive Molecules Kupffer Cells
DOI10.1016/j.yexcr.2017.01.002
Indexed BySCI
Language英语
WOS IDWOS:000394196700011
WOS KeywordBLOOD-BRAIN-BARRIER ; INFLAMED LIVER ; IN-VITRO ; HEPATIC MICROCIRCULATION ; MOLECULE-1 EXPRESSION ; LEUKOCYTE RECRUITMENT ; EXTRACELLULAR-MATRIX ; ISCHEMIA-REPERFUSION ; STERILE INFLAMMATION ; INJURY
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
Funding OrganizationNational Natural Science Foundation of China(31230027 ; Frontier Science Key Project of Chinese Academy of Sciences(QYZDJ-SSW-JSC018 ; Strategic Priority Research Program of Chinese Academy of Sciences(QYZDJ-SSW-JSC018 ; 91642203 ; XDB22040101) ; XDB22040101) ; 31661143044 ; 31110103918 ; 31300776)
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类
Ranking1
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/60576
Collection国家微重力实验室
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Yang H,Li N,Du Y,et al. Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow[J]. EXPERIMENTAL CELL RESEARCH,2017,351(1):91-99.
APA 杨浩.,李宁.,杜宇.,佟春芳.,吕守芹.,...&Long, M .(2017).Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow.EXPERIMENTAL CELL RESEARCH,351(1),91-99.
MLA 杨浩,et al."Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow".EXPERIMENTAL CELL RESEARCH 351.1(2017):91-99.
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