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Mechanical remodeling of normally sized mammalian cells under a gravity vector
Zhang C; Zhou LW(周吕文); Zhang F(张帆); Lv DY(吕东媛); Li N(李宁); Zheng L; Xu YH(徐艳红); Li Z; Sun SJ(孙树津); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.
Source PublicationFASEB JOURNAL
2017-02-01
Volume31Issue:2Pages:802-813
ISSN0892-6638
AbstractTranslocation of the dense nucleus along a gravity vector initiates mechanical remodeling of a cell but the underlying mechanisms of cytoskeletal network and focal adhesion complex (FAC) reorganization in a mammalian cell remain unclear. We quantified the remodeling of an MC3T3-E1 cell placed in upward- downward- or edge-on-orientated substrate. Nucleus longitudinal translocation presents a high value in downward orientation at 24 h or in edge-on orientation at 72 h which is consistent with orientation-dependent distribution of perinuclear actin stress fibers and vimentin cords. Redistribution of total FAC area and fractionized super mature adhesion number coordinates this dependence at short duration. This orientation-dependent remodeling is associated with nucleus flattering and lamin A/C phosphorylation. Actin depolymerization or Rho-associated protein kinase signaling inhibition abolishes the orientation dependence of nucleus translocation whereas tubulin polymerization inhibition or vimentin disruption reserves the dependence. A biomechanical model is therefore proposed for integrating the mechanosensing of nucleus translocation with cytoskeletal remodeling and FAC reorganization induced by a gravity vector.
KeywordGravity-directed Mechanosensing Nucleus Translocation Cytoskeletal Remodeling Fac Reorganization
DOI10.1096/fj.201600897RR
Indexed BySCI
Language英语
WOS IDWOS:000394235400032
WOS KeywordFOCAL ADHESION DYNAMICS ; ROS 17/2.8 CELLS ; NANOSCALE ARCHITECTURE ; ACTIN ; FORCE ; MECHANOTRANSDUCTION ; CYTOSKELETON ; STRESS ; TALIN ; MICROTUBULES
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biology ; Cell Biology
Funding OrganizationNational Key Basic Research Foundation of China(2011CB710904) ; Strategic Priority Research Program(XDA04020219 ; National Natural Science Foundation of China(31110103918) ; National High Technology Research and Development Program of China(2011AA020109) ; XDA01030102)
DepartmentNML分子-细胞生物力学与空间生命科学
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/60581
Collection国家微重力实验室
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Inst Mech, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Zhang C,Zhou LW,Zhang F,et al. Mechanical remodeling of normally sized mammalian cells under a gravity vector[J]. FASEB JOURNAL,2017,31(2):802-813.
APA Zhang C.,周吕文.,张帆.,吕东媛.,李宁.,...&Long, M .(2017).Mechanical remodeling of normally sized mammalian cells under a gravity vector.FASEB JOURNAL,31(2),802-813.
MLA Zhang C,et al."Mechanical remodeling of normally sized mammalian cells under a gravity vector".FASEB JOURNAL 31.2(2017):802-813.
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