| Mechanical remodeling of normally sized mammalian cells under a gravity vector |
| Zhang C ; Zhou LW(周吕文); Zhang F(张帆); Lv DY(吕东媛); Li N(李宁); Zheng L; Xu YH(徐艳红); Li Z; Sun SJ(孙树津); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.
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| 发表期刊 | FASEB JOURNAL
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| 2017-02-01
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| 卷号 | 31期号:2页码:802-813 |
| ISSN | 0892-6638
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| 摘要 | Translocation of the dense nucleus along a gravity vector initiates mechanical remodeling of a cell but the underlying mechanisms of cytoskeletal network and focal adhesion complex (FAC) reorganization in a mammalian cell remain unclear. We quantified the remodeling of an MC3T3-E1 cell placed in upward- downward- or edge-on-orientated substrate. Nucleus longitudinal translocation presents a high value in downward orientation at 24 h or in edge-on orientation at 72 h which is consistent with orientation-dependent distribution of perinuclear actin stress fibers and vimentin cords. Redistribution of total FAC area and fractionized super mature adhesion number coordinates this dependence at short duration. This orientation-dependent remodeling is associated with nucleus flattering and lamin A/C phosphorylation. Actin depolymerization or Rho-associated protein kinase signaling inhibition abolishes the orientation dependence of nucleus translocation whereas tubulin polymerization inhibition or vimentin disruption reserves the dependence. A biomechanical model is therefore proposed for integrating the mechanosensing of nucleus translocation with cytoskeletal remodeling and FAC reorganization induced by a gravity vector. |
| 关键词 | Gravity-directed
Mechanosensing
Nucleus Translocation
Cytoskeletal Remodeling
Fac Reorganization
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| DOI | 10.1096/fj.201600897RR
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| 收录类别 | SCI
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| 语种 | 英语
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| WOS记录号 | WOS:000394235400032
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| 关键词[WOS] | FOCAL ADHESION DYNAMICS
; ROS 17/2.8 CELLS
; NANOSCALE ARCHITECTURE
; ACTIN
; FORCE
; MECHANOTRANSDUCTION
; CYTOSKELETON
; STRESS
; TALIN
; MICROTUBULES
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| WOS研究方向 | Biochemistry & Molecular Biology
; Life Sciences & Biomedicine - Other Topics
; Cell Biology
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| WOS类目 | Biochemistry & Molecular Biology
; Biology
; Cell Biology
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| 项目资助者 | National Key Basic Research Foundation of China(2011CB710904)
; Strategic Priority Research Program(XDA04020219
; National Natural Science Foundation of China(31110103918)
; National High Technology Research and Development Program of China(2011AA020109)
; XDA01030102)
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| 课题组名称 | NML分子-细胞生物力学与空间生命科学
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| 论文分区 | 一类
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| 力学所作者排名 | 1
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/60581
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| 专题 | 微重力重点实验室
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| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, 15 North 4th Ring Rd, Beijing 100190, Peoples R China. |
推荐引用方式
GB/T 7714 |
Zhang C,Zhou LW,Zhang F,et al. Mechanical remodeling of normally sized mammalian cells under a gravity vector[J]. FASEB JOURNAL,2017,31,2,:802-813.
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| APA |
Zhang C.,周吕文.,张帆.,吕东媛.,李宁.,...&Long, M .(2017).Mechanical remodeling of normally sized mammalian cells under a gravity vector.FASEB JOURNAL,31(2),802-813.
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| MLA |
Zhang C,et al."Mechanical remodeling of normally sized mammalian cells under a gravity vector".FASEB JOURNAL 31.2(2017):802-813.
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