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Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation
Li N(李宁); Mao DB(毛德斌); Gong YX(龚一心); Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
Source PublicationChinese Journal of Biomedical Engineering
2015
Volume24Issue:1Pages:40-46
AbstractLFA-1 and Mac-1, two β2integrin members constitutively expressed on neutrophils, mediate leukocyte recruitment cascade by binding to the same ligand of ICAM-1. The slow rolling and firm adhesion of leukocytes rely on LFA-1 while the cell crawling is dependent on Mac-1. We hypothesized that their distinct roles are likely attributed to the differences in the binding kinetics or in the diverse responses of outside-in and inside-out signaling. In this study, we compared the ICAM-1 binding features between soluble or membrane-expressed LFA-1 and Mac-1 with different affinity conformation using optical trap technique. Our data indicated that the affinity up-regulation from wide type(WT) to high affinity(HA) is off-rate dependent for LFA-1but on-rate dependent for Mac-1. The structural bases of this new finding were found to be consistent with our previous simulations. These results furthered our understanding in their function differences under shear flow.
Language英语
DepartmentNML分子-细胞生物力学与空间生命科学
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/60641
Collection国家微重力实验室
Recommended Citation
GB/T 7714
Li N,Mao DB,Gong YX,et al. Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation[J]. Chinese Journal of Biomedical Engineering,2015,24(1):40-46.
APA Li N,Mao DB,Gong YX,Lv SQ,Zhang Y,&Long M.(2015).Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation.Chinese Journal of Biomedical Engineering,24(1),40-46.
MLA Li N,et al."Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation".Chinese Journal of Biomedical Engineering 24.1(2015):40-46.
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