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Conformational folding and disulfide bonding drive distinct stages of protein structure formation
Lv JM; Lv SQ(吕守芹); Liu ZP; Zhang J; Gao BoX; Yao ZY; Wu YX; Potempa LA.; Ji SR; Long M(龙勉); Wu Y
发表期刊SCIENTIFIC REPORTS
2018-01-24
卷号8页码:1494
ISSN2045-2322
摘要

The causal relationship between conformational folding and disulfide bonding in protein oxidative folding remains incompletely defined. Here we show a stage-dependent interplay between the two events in oxidative folding of C-reactive protein (CRP) in live cells. CRP is composed of five identical subunits, which first fold spontaneously to a near-native core with a correctly positioned C-terminal helix. This process drives the formation of the intra-subunit disulfide bond between Cys36 and Cys97. The second stage of subunit folding, however, is a non-spontaneous process with extensive restructuring driven instead by the intra-subunit disulfide bond and guided by calcium binding-mediated anchoring. With the folded subunits, pentamer assembly ensues. Our results argue that folding spontaneity is the major determinant that dictates which event acts as the driver. The stepwise folding pathway of CRP further suggests that one major route might be selected out of the many in theory for efficient folding in the cellular environment.

DOI10.1038/s41598-018-20014-y
收录类别SCI ; EI
语种英语
WOS记录号WOS:000423154000024
关键词[WOS]C-reactive-protein ; Molecular-dynamics ; Expression ; Calcium ; Binding ; Form ; Crp
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
项目资助者NSFC(31470718 ; Fundamental Research Funds for the Central Universities(lzujbky-2016-k11 ; 31570749 ; lzujbky-2016-222) ; 31230027 ; 91642203)
论文分区二类/q1
力学所作者排名1
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/77463
专题国家微重力实验室
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GB/T 7714
Lv JM,Lv SQ,Liu ZP,et al. Conformational folding and disulfide bonding drive distinct stages of protein structure formation[J]. SCIENTIFIC REPORTS,2018,8:1494.
APA Lv JM.,Lv SQ.,Liu ZP.,Zhang J.,Gao BoX.,...&Wu Y.(2018).Conformational folding and disulfide bonding drive distinct stages of protein structure formation.SCIENTIFIC REPORTS,8,1494.
MLA Lv JM,et al."Conformational folding and disulfide bonding drive distinct stages of protein structure formation".SCIENTIFIC REPORTS 8(2018):1494.
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