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Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling
Li N(李宁); Yang H(杨浩); Wang ML; Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
2018-02-15
发表期刊MOLECULAR BIOLOGY OF THE CELL
卷号29期号:4页码:408-418
ISSN1059-1524
摘要Lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) and their counterreceptors such as intercellular cell adhesion molecules (ICAM-1 and ICAM-2), junctional adhesion molecules (JAM-A, JAM-C), and receptors for advanced glycation end products (RAGE) are crucial for promoting polymorphonuclear leukocyte (neutrophil, PMN) recruitment. The underlying mechanisms of ligand-specific bindings in this cascade remain incompletely known. We compared the dynamic force spectra for various LFA-1/Mac1-ligand bonds using single-molecule atomic force microscopy (AFM) and tested their functions in mediating PMN recruitment under in vitro shear flow. Distinct features of bond rupture forces and lifetimes were uncovered for these ligands, implying their diverse roles in regulating PMN adhesion on endothelium. LFA-1 dominates PMN adhesion on ICAM-1 and ICAM-2, while Mac-1 mediates PMN adhesion on RAGE, JAM-A, and JAM-C, which is consistent with their bond strength. All ligands can trigger PMN spreading and polarization, in which Mac-1 seems to induce outside-in signaling more effectively. LFA-1-ICAM-1 and LFA-1/Mac-1-JAM-C bonds can accelerate PMN crawling under high shear stress, presumably due to their high mechanical strength. This work provides new insight into basic molecular mechanisms of physiological ligands of beta 2 integrins in PMN recruitment.
DOI10.1091/mbc.E16-12-0827
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收录类别SCI
语种英语
WOS记录号WOS:000425859100005
关键词[WOS]INFLAMMATORY CELL RECRUITMENT ; HIGH-STRENGTH STATES ; BETA(2) INTEGRIN ; ENDOTHELIAL-CELLS ; SHEAR-FLOW ; IN-VIVO ; TRANSENDOTHELIAL MIGRATION ; CONFORMATIONAL-CHANGES ; LEUKOCYTE RECRUITMENT ; MOLECULE-1 ICAM-1
WOS研究方向Cell Biology
WOS类目Cell Biology
项目资助者National Natural Science Foundation of China [31230027, 31110103918, 31300776] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences [XDA01030102, XDB22040101, QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China [2016YFA0501601] ; Visiting Scholar Foundation of the Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education [CQKLBST-2015-002]
论文分区二类
力学所作者排名1
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/77801
专题国家微重力实验室
作者单位1.Chinese Acad Sci, Ctr Biomech & Bioengn, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
4.Chongqing Univ, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China
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GB/T 7714
Li N,Yang H,Wang ML,et al. Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling[J]. MOLECULAR BIOLOGY OF THE CELL,2018,29(4):408-418.
APA 李宁,杨浩,Wang ML,吕守芹,章燕,&龙勉.(2018).Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.MOLECULAR BIOLOGY OF THE CELL,29(4),408-418.
MLA 李宁,et al."Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling".MOLECULAR BIOLOGY OF THE CELL 29.4(2018):408-418.
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