IMECH-IR  > 国家微重力实验室
Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling
Li N(李宁); Yang H(杨浩); Wang ML; Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
Source PublicationMOLECULAR BIOLOGY OF THE CELL
2018-02-15
Volume29Issue:4Pages:408-418
ISSN1059-1524
AbstractLymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) and their counterreceptors such as intercellular cell adhesion molecules (ICAM-1 and ICAM-2), junctional adhesion molecules (JAM-A, JAM-C), and receptors for advanced glycation end products (RAGE) are crucial for promoting polymorphonuclear leukocyte (neutrophil, PMN) recruitment. The underlying mechanisms of ligand-specific bindings in this cascade remain incompletely known. We compared the dynamic force spectra for various LFA-1/Mac1-ligand bonds using single-molecule atomic force microscopy (AFM) and tested their functions in mediating PMN recruitment under in vitro shear flow. Distinct features of bond rupture forces and lifetimes were uncovered for these ligands, implying their diverse roles in regulating PMN adhesion on endothelium. LFA-1 dominates PMN adhesion on ICAM-1 and ICAM-2, while Mac-1 mediates PMN adhesion on RAGE, JAM-A, and JAM-C, which is consistent with their bond strength. All ligands can trigger PMN spreading and polarization, in which Mac-1 seems to induce outside-in signaling more effectively. LFA-1-ICAM-1 and LFA-1/Mac-1-JAM-C bonds can accelerate PMN crawling under high shear stress, presumably due to their high mechanical strength. This work provides new insight into basic molecular mechanisms of physiological ligands of beta 2 integrins in PMN recruitment.
DOI10.1091/mbc.E16-12-0827
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000425859100005
WOS KeywordINFLAMMATORY CELL RECRUITMENT ; HIGH-STRENGTH STATES ; BETA(2) INTEGRIN ; ENDOTHELIAL-CELLS ; SHEAR-FLOW ; IN-VIVO ; TRANSENDOTHELIAL MIGRATION ; CONFORMATIONAL-CHANGES ; LEUKOCYTE RECRUITMENT ; MOLECULE-1 ICAM-1
WOS Research AreaCell Biology
WOS SubjectCell Biology
Funding OrganizationNational Natural Science Foundation of China [31230027, 31110103918, 31300776] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences [XDA01030102, XDB22040101, QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China [2016YFA0501601] ; Visiting Scholar Foundation of the Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education [CQKLBST-2015-002]
Classification二类
Ranking1
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/77801
Collection国家微重力实验室
Affiliation1.Chinese Acad Sci, Ctr Biomech & Bioengn, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
4.Chongqing Univ, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China
Recommended Citation
GB/T 7714
Li N,Yang H,Wang ML,et al. Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling[J]. MOLECULAR BIOLOGY OF THE CELL,2018,29(4):408-418.
APA 李宁,杨浩,Wang ML,吕守芹,章燕,&龙勉.(2018).Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.MOLECULAR BIOLOGY OF THE CELL,29(4),408-418.
MLA 李宁,et al."Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling".MOLECULAR BIOLOGY OF THE CELL 29.4(2018):408-418.
Files in This Item: Download All
File Name/Size DocType Version Access License
IrJ2018186.pdf(1832KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[李宁]'s Articles
[杨浩]'s Articles
[Wang ML]'s Articles
Baidu academic
Similar articles in Baidu academic
[李宁]'s Articles
[杨浩]'s Articles
[Wang ML]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[李宁]'s Articles
[杨浩]'s Articles
[Wang ML]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: IrJ2018186.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.