Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin | |
Li YK; Liu W(刘巍); Jin G(靳刚); Niu Y(牛宇); Chen YP; Xie MX | |
Source Publication | ANALYTICAL CHEMISTRY |
2018-07-03 | |
Volume | 90Issue:13Pages:8002-8010 |
ISSN | 0003-2700 |
Abstract | Analysis of trace low molecular weight (LMW) proteins in serum using the label-free imaging ellipsometry (IE) immunosensor is still a challenge due to the lack of an effective signal amplification strategy and the serious nonspecific adsorption. Herein we have developed a sandwich strategy mediated IE (SSIE) immunosensor to enable the immunodetection of LMW protein biomarkers in serum samples. We have first found that the weak binding affinity and the insufficient surface amount density of the ligand are two important factors which hinder the detection of LMW proteins in serum using the IE immunosensor. Then we have deduced that the sandwich strategy can amplify the detection signal of IE and avoid the nonspecific adsorption in serum. As a validation of the serological detection of LMW proteins, the SSIE immunosensor has been used to accomplish the quantitative detection of procalcitonin (PCT) in serum. Compared with other PCT analysis methodologies, the SSIE immunosensor enjoys the advantages of simplicity, rapidity, and sufficient sensitivity. Furthermore, we have proposed the criteria to predict the ability of the SSIE immunosensor for the detection of LMW protein biomarkers in serum, which can make the detection of LMW proteins smart and efficient. |
DOI | 10.1021/acs.analchem.8b00888 |
URL | 查看原文 |
Indexed By | SCI ; EI |
Language | 英语 |
WOS ID | WOS:000438008600031 |
WOS Keyword | Sepsis Diagnosis ; Tumor-markers ; Immunoassays ; Biosensor ; Adsorption ; Complex ; Sensor |
WOS Research Area | Chemistry, Analytical |
WOS Subject | Chemistry |
Funding Organization | International Science and Technology Cooperation Program of China [2015DFG32390] ; National Basic Research Program of China [2015CB352100] ; National Natural Science Foundation of China [11704218, 81472941, 21305147, 81671784] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2017026] |
Classification | 一类 |
Ranking | 1 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://dspace.imech.ac.cn/handle/311007/77828 |
Collection | 微重力重点实验室 |
Corresponding Author | Niu Y(牛宇); Chen YP; Xie MX |
Affiliation | 1.Beijing Normal Univ, Analyt & Testing Ctr, Beijing 100875, Peoples R China 2.Tsinghua Univ, Inst Microelect, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engineered Construct & Mechanobio, NML, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 5.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
Recommended Citation GB/T 7714 | Li YK,Liu W,Jin G,et al. Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin[J]. ANALYTICAL CHEMISTRY,2018,90,13,:8002-8010. |
APA | Li YK,Liu W,Jin G,Niu Y,Chen YP,&Xie MX.(2018).Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin.ANALYTICAL CHEMISTRY,90(13),8002-8010. |
MLA | Li YK,et al."Label-Free Sandwich Imaging Ellipsometry Immunosensor for Serological Detection of Procalcitonin".ANALYTICAL CHEMISTRY 90.13(2018):8002-8010. |
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