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Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations
Li N(李宁); Zhang X(张潇); Li PW; Yang H; Tong CF; Lv SQ(吕守芹); Zhang Y(章燕); Ye ZY; Pan J; Long M(龙勉)
Source PublicationCMES-COMPUTER MODELING IN ENGINEERING & SCIENCES
2018-08-01
Volume116Issue:2Pages:263-279
ISSN1526-1492
AbstractNeutrophil (PMN) accumulation on liver sinusoidal endothelial cells (LSECs) is crucial to pathogen clearance and tissue damage in the liver sinusoids and controlled by a series of adhesion molecules expressed on the surface of PMNs and LSECs. The role of lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) in this process is still contentious. Here we compared the dynamic force spectra of the binding of beta(2) integrin to intercellular adhesion molecule-1 (ICAM-1) on LSECs using atomic force microscopy (AFM) and performed free and steered molecular dynamics (MD) simulations to analyze their structural bases of LFA-1- or Mac-1-I-domain and ICAM-1-D1 or D3 pair in their force spectra. Our AFM data suggest that the mechanical strength of LFA-1-ICAM-1 bond is significantly stronger than that of Mac-I-ICAM-1 bond, implying a dominate role for LFA-1 to mediate PMN adhesion under shear flow. MD simulations indicated that spontaneous dissociation of Mac-1-I-domain vs. ICAM-D3-domain is slower with the stronger interaction energy than that for LFA-1 I-domain vs. ICAM-D1-domain and that the rupture force for Mac-1 is lower than that for LFA-1, which are in qualitative agreement with the above experimental observations. These data indicate that the biomechanical features of LFA-1 and Mac-1 to mediate PMN adhesion on LSECs in vitro are similar with those in other tissues like cerebrovascular endothelium, while Mac-1-mediated PMN recruitment in liver sinusoids may stem from the slow blood flow in vivo. These findings further the understandings of PMN recruitment under shear flow in liver sinusoids.
KeywordLiver sinusoidal endothelial cells neutrophils beta(2) integrin shear flow mechanical strength
DOI10.31614/cmes.2018.04079
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000444791300011
WOS KeywordHEPATIC MICROCIRCULATION ; ICAM-1 BONDS ; I-DOMAIN ; ADHESION ; LFA-1 ; MAC-1 ; INFLAMMATION ; SPECTROSCOPY ; MIGRATION ; BINDING
WOS Research AreaEngineering, Multidisciplinary ; Mathematics, Interdisciplinary Applications
WOS SubjectEngineering ; Mathematics
Funding OrganizationNational Key Research and Development Program of China [2016YFA0501601] ; National Natural Science Foundation of China [31661143044, 31300776] ; Strategic Priority Research Program of Chinese Academy of Sciences [XDB22040101, QYZDJ-SSW-JSC018] ; Visiting Scholar Foundation of the Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education [CQKLBST-2015-002] ; Frontier Science Key Project of Chinese Academy of Sciences [XDB22040101, QYZDJ-SSW-JSC018]
ClassificationQ4
Ranking1
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/77844
Collection国家微重力实验室
Affiliation1.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Ctr Biomech & Bioengn, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, 174 Shazhengjie, Chongqing 400044, Peoples R China
Recommended Citation
GB/T 7714
Li N,Zhang X,Li PW,et al. Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations[J]. CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES,2018,116(2):263-279.
APA 李宁.,张潇.,Li PW.,Yang H.,Tong CF.,...&龙勉.(2018).Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations.CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES,116(2),263-279.
MLA 李宁,et al."Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations".CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES 116.2(2018):263-279.
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