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Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations
Li N(李宁); Zhang X(张潇); Li PW; Yang H; Tong CF; Lv SQ(吕守芹); Zhang Y(章燕); Ye ZY; Pan J; Long M(龙勉)
2018-08-01
发表期刊CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES
卷号116期号:2页码:263-279
ISSN1526-1492
摘要Neutrophil (PMN) accumulation on liver sinusoidal endothelial cells (LSECs) is crucial to pathogen clearance and tissue damage in the liver sinusoids and controlled by a series of adhesion molecules expressed on the surface of PMNs and LSECs. The role of lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) in this process is still contentious. Here we compared the dynamic force spectra of the binding of beta(2) integrin to intercellular adhesion molecule-1 (ICAM-1) on LSECs using atomic force microscopy (AFM) and performed free and steered molecular dynamics (MD) simulations to analyze their structural bases of LFA-1- or Mac-1-I-domain and ICAM-1-D1 or D3 pair in their force spectra. Our AFM data suggest that the mechanical strength of LFA-1-ICAM-1 bond is significantly stronger than that of Mac-I-ICAM-1 bond, implying a dominate role for LFA-1 to mediate PMN adhesion under shear flow. MD simulations indicated that spontaneous dissociation of Mac-1-I-domain vs. ICAM-D3-domain is slower with the stronger interaction energy than that for LFA-1 I-domain vs. ICAM-D1-domain and that the rupture force for Mac-1 is lower than that for LFA-1, which are in qualitative agreement with the above experimental observations. These data indicate that the biomechanical features of LFA-1 and Mac-1 to mediate PMN adhesion on LSECs in vitro are similar with those in other tissues like cerebrovascular endothelium, while Mac-1-mediated PMN recruitment in liver sinusoids may stem from the slow blood flow in vivo. These findings further the understandings of PMN recruitment under shear flow in liver sinusoids.
关键词Liver sinusoidal endothelial cells neutrophils beta(2) integrin shear flow mechanical strength
DOI10.31614/cmes.2018.04079
URL查看原文
收录类别SCI ; EI
语种英语
WOS记录号WOS:000444791300011
关键词[WOS]HEPATIC MICROCIRCULATION ; ICAM-1 BONDS ; I-DOMAIN ; ADHESION ; LFA-1 ; MAC-1 ; INFLAMMATION ; SPECTROSCOPY ; MIGRATION ; BINDING
WOS研究方向Engineering, Multidisciplinary ; Mathematics, Interdisciplinary Applications
WOS类目Engineering ; Mathematics
项目资助者National Key Research and Development Program of China [2016YFA0501601] ; National Natural Science Foundation of China [31661143044, 31300776] ; Strategic Priority Research Program of Chinese Academy of Sciences [XDB22040101, QYZDJ-SSW-JSC018] ; Visiting Scholar Foundation of the Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education [CQKLBST-2015-002] ; Frontier Science Key Project of Chinese Academy of Sciences [XDB22040101, QYZDJ-SSW-JSC018]
论文分区Q4
力学所作者排名1
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/77844
专题国家微重力实验室
作者单位1.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Ctr Biomech & Bioengn, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, 174 Shazhengjie, Chongqing 400044, Peoples R China
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Li N,Zhang X,Li PW,et al. Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations[J]. CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES,2018,116(2):263-279.
APA 李宁.,张潇.,Li PW.,Yang H.,Tong CF.,...&龙勉.(2018).Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations.CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES,116(2),263-279.
MLA 李宁,et al."Mechanical Strength and Structural Basis of beta(2) Integrin to Mediate Neutrophil Accumulation on Liver Sinusoidal Endothelial Cells: A Study Using Atomic Force Microscopy and Molecular Dynamics Simulations".CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES 116.2(2018):263-279.
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