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Theoretical modeling of mechanical homeostasis of a mammalian cell under gravity-directed vector
Zhou LW(周吕文); Zhang C(张晨); Zhang F(张芳); Lv SQ(吕守芹); Sun SJ(孙树津); Lv DY(吕东媛); Long M(龙勉)
Source PublicationBIOMECHANICS AND MODELING IN MECHANOBIOLOGY
2018-02-01
Volume17Issue:1Pages:191-203
ISSN1617-7959
AbstractTranslocation of dense nucleus along gravity vector initiates mechanical remodeling of a eukaryotic cell. In our previous experiments, we quantified the impact of gravity vector on cell remodeling by placing an MC3T3-E1 cell onto upward (U)-, downward (D)-, or edge-on (E)- orientated substrate. Our experimental data demonstrate that orientation dependence of nucleus longitudinal translocation is positively correlated with cytoskeletal (CSK) remodeling of their expressions and structures and also is associated with rearrangement of focal adhesion complex (FAC). However, the underlying mechanism how CSK network and FACs are reorganized in a mammalian cell remains unclear. In this paper, we developed a theoretical biomechanical model to integrate the mechanosensing of nucleus translocation with CSK remodeling and FAC reorganization induced by a gravity vector. The cell was simplified as a nucleated tensegrity structure in the model. The cell and CSK filaments were considered to be symmetrical. All elements of CSK filaments and cytomembrane that support the nucleus were simplified as springs. FACs were simplified as an adhesion cluster of parallel bonds with shared force. Our model proposed that gravity vector-directed translocation of the cell nucleus is mechanically balanced by CSK remodeling and FAC reorganization induced by a gravitational force. Under gravity, dense nucleus tends to translocate and exert additional compressive or stretching force on the cytoskeleton. Finally, changes of the tension force acting on talin by microfilament alter the size of FACs. Results from our model are in qualitative agreement with those from experiments.
KeywordGravity directed Mechanosensing Nucleus translocation Cytoskeletal remodeling FAC reorganization
DOI10.1007/s10237-017-0954-y
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000424651600014
WOS KeywordINTERMEDIATE-FILAMENTS ; FOCAL ADHESIONS ; ADHERENT CELLS ; CYTOSKELETON ; MECHANOTRANSDUCTION ; MICROGRAVITY ; ARCHITECTURE ; FORCE
WOS Research AreaBiophysics ; Engineering, Biomedical
WOS SubjectBiophysics ; Engineering
Funding OrganizationStrategic Priority Research Program of Chinese Science Academy of Sciences [XDA04020219] ; National Natural Science Foundation of China [31110103918] ; National Key Basic Research Foundation of China [2011CB710904]
Classification二类
Ranking1
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/77879
Collection国家微重力实验室
Affiliation1.Chinese Acad Sci, Ctr Biomech & Bioengn, Lab Micrograv, Natl Micrograv Lab, Beijing, Peoples R China
2.Chinese Acad Sci, Beijing Key Lab Engn Construct & Mech, Inst Mech, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhou LW,Zhang C,Zhang F,et al. Theoretical modeling of mechanical homeostasis of a mammalian cell under gravity-directed vector[J]. BIOMECHANICS AND MODELING IN MECHANOBIOLOGY,2018,17(1):191-203.
APA 周吕文.,张晨.,张芳.,吕守芹.,孙树津.,...&龙勉.(2018).Theoretical modeling of mechanical homeostasis of a mammalian cell under gravity-directed vector.BIOMECHANICS AND MODELING IN MECHANOBIOLOGY,17(1),191-203.
MLA 周吕文,et al."Theoretical modeling of mechanical homeostasis of a mammalian cell under gravity-directed vector".BIOMECHANICS AND MODELING IN MECHANOBIOLOGY 17.1(2018):191-203.
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