IMECH-IR  > 国家微重力实验室
Liver sinusoid on a chip
Du Y(杜宇)1,2; Li N(李宁)1,2,3; Long M(龙勉)1,2,3
Corresponding AuthorLong, Mian(mlong@imech.ac.cn)
Source PublicationMICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS
2018
Volume146Pages:105-134
ISSN0091-679X
AbstractLiver sinusoid is the main functional site in liver. Multiple types of hepatic cells are well organized in a precisely-controlled biochemical and biomechanical environment, maintaining a spectrum of hepatic functions. Here, using micro-engineering techniques, four types of primary hepatic cells are integrated into two layer channels connected by porous membrane, which recreates the sinusoidal cell composition and architecture. By incorporating shear flow into this permeable system, the blood flow in sinusoids and interstitial flow in space of Disse are recapitulated. Conventional hepatocyte-based liver-specific functions are enhanced by non-parenchymal cells co-culture and shear flow. Moreover, major immune responses in liver sinusoids, i.e., neutrophil recruitment under lipopolysaccharide (LPS) stimulation, are replicated, indicating that all types of hepatic cells contribute to this process. Thus, this liver chip provides a new in vitro model to investigate the short-duration cellular interactions under a microenvironment mimicking the physiological composition and architecture of liver organ.
DOI10.1016/bs.mcb.2018.06.002
Indexed BySCI
Language英语
WOS IDWOS:000452411700007
WOS KeywordENDOTHELIAL-CELLS ; INFLAMED LIVER ; KUPFFER CELLS ; GENE-EXPRESSION ; CXC CHEMOKINES ; FLOW DYNAMICS ; INJURY ; NEUTROPHILS ; MECHANISMS ; LYMPHOCYTES
WOS Research AreaCell Biology
WOS SubjectCell Biology
ClassificationQ4
Ranking1
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/78962
Collection国家微重力实验室
Affiliation1.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing, Peoples R China;
2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing, Peoples R China;
3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Du Y,Li N,Long M. Liver sinusoid on a chip[J]. MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS,2018,146:105-134.
APA 杜宇,李宁,&龙勉.(2018).Liver sinusoid on a chip.MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS,146,105-134.
MLA 杜宇,et al."Liver sinusoid on a chip".MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS 146(2018):105-134.
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