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An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite
Sun SJ(孙树津); Wang CZ(王成之); Bi YZ(毕于振); Li N(李宁); Lv DY(吕东媛); Chen Q(陈琴); Chen J(陈娟); Long M(龙勉)
Source PublicationREVIEW OF SCIENTIFIC INSTRUMENTS
2019
Volume90Issue:7Pages:12
ISSN0034-6748
Abstract

Pathophysiological changes of astronauts under space microgravity involve complex factors and require an integrative perspective to fully understand the mechanisms. The readouts from space cell biology experiments strongly depend on the hardware and especially the cell bioreactor that is used in distinct spacecraft. Herein, a specialized cell culture bioreactor is designed for culturing mammalian cells on board the SJ-10 satellite. This hardware focuses mainly on satisfying the requirements of gas exchange, bubble separation, and flow control, as well as their functional and structural integration on cell culture within the technical and environmental constraints of the spacecraft platform under microgravity. A passive bubble separator is constructed and is connected in series to an individual cell culture chamber to remove the bubbles that were produced in orbit during cell growth. A moderate flow rate is preset to provide sufficient mass transfer and low shear stress in a well-designed flow circuit. Together with other modules of temperature control, in situ microscopic imaging, and online imaging acquisition, this novel space cell culture system is successfully used to culture human endothelial cells and rat bone marrow-derived mesenchymal stem cells in the SJ-10 mission. The advantages and shortcomings of the integration design are discussed for this type of the hardware.

DOI10.1063/1.5087770
Indexed BySCI ; EI
Language英语
WOS IDWOS:000478661400017
WOS KeywordMAMMALIAN-CELL ; BIOREACTOR ; MORPHOLOGY
WOS Research AreaInstruments & Instrumentation ; Physics
WOS SubjectInstruments & Instrumentation ; Physics, Applied
Funding ProjectNational Natural Science Foundation of China[U1738115] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDA04020202-17] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDA04020416] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDA04073800] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China[2016YFA0501601]
Funding OrganizationNational Natural Science Foundation of China ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences ; National Key Research and Development Program of China
ClassificationQ3
Ranking1
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/79495
Collection国家微重力实验室
Recommended Citation
GB/T 7714
Sun SJ,Wang CZ,Bi YZ,et al. An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2019,90(7):12.
APA 孙树津.,王成之.,毕于振.,李宁.,吕东媛.,...&龙勉.(2019).An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite.REVIEW OF SCIENTIFIC INSTRUMENTS,90(7),12.
MLA 孙树津,et al."An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite".REVIEW OF SCIENTIFIC INSTRUMENTS 90.7(2019):12.
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