IMECH-IR  > 非线性力学国家重点实验室
Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis
Yun JL1; Zheng XW1; Xu P1,6; Zheng X(郑旭)2; Xu JY1,3; Cao C4,7; Fu YS4; Xu BX1,3; Dai X1; Wang Y1; Liu HT1,3; Yi QL1,3; Zhu YX1; Wang J1; Wang L1; Dong ZY1,3; Huang L1,3; Huang YY4; Du WB1,3,5
Corresponding AuthorHuang, Li(huangl@im.ac.cn) ; Huang, Yanyi(huang@pku.edu.cn) ; Du, Wenbin(wenbin@im.ac.cn)
Source PublicationSMALL
2019-09-29
Pages8
ISSN1613-6810
AbstractSingle-cell analysis offers unprecedented resolution for the investigation of cellular heterogeneity and the capture of rare cells from large populations. Here, described is a simple method named interfacial nanoinjection (INJ), which can miniaturize various single-cell assays to be performed in nanoliter water-in-oil droplets on standard microwell plates. The INJ droplet handler can adjust droplet volumes for multistep reactions on demand with high precision and excellent monodispersity, and consequently enables a wide range of single-cell assays. Importantly, INJ can be coupled with fluorescence-activated cell sorting (FACS), which is currently the most effective and accurate single-cell sorting and isolation method. FACS-INJ pipelines for high-throughput plate well-based single-cell analyses, including single-cell proliferation, drug-resistance testing, polymerase chain reaction (PCR), reverse-transcription PCR, and whole-genome sequencing are introduced. This FACS-INJ pipeline is compatible with a wide range of samples and can be extended to various single-cell analysis applications in microbiology, cell biology, and biomedical diagnostics.
Keyworddroplet fluorescence-activated cell sorting microfluidics single-cell analysis single-cell sequencing
DOI10.1002/smll.201903739
Indexed BySCI ; EI
Language英语
WOS IDWOS:000488077200001
WOS KeywordHIGH-THROUGHPUT ; AMPLIFICATION ; TECHNOLOGIES ; MICROBES ; REVEALS ; GENOMES ; DISPLAY
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
Funding ProjectChina Ocean Mineral Resources RD Association[DY135-B2-02] ; National Natural Science Foundation of China[41977196] ; National Natural Science Foundation of China[21822408] ; National Natural Science Foundation of China[21525521] ; National Natural Science Foundation of China[11832017] ; National Natural Science Foundation of China[11572335] ; National Key R&D Program of China[2016YFE0205800] ; National Key R&D Program of China[2016YFC0100900] ; National Key R&D Program of China[2018YFC0310703] ; Chinese Academy of Sciences[XDB15040102] ; Chinese Academy of Sciences[QYZDB-SSW-SMC008] ; Chinese Academy of Sciences[QYZDB-SSW-JSC036] ; Chinese Academy of Sciences[KFZD-SW-219-4] ; Chinese Academy of Sciences[XDB22040403] ; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences[SKLMR-20150602]
Funding OrganizationChina Ocean Mineral Resources RD Association ; National Natural Science Foundation of China ; National Key R&D Program of China ; Chinese Academy of Sciences ; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Classification一类
Ranking4
ContributorHuang, Li ; Huang, Yanyi ; Du, Wenbin
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/80644
Collection非线性力学国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China;
2.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China;
3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China;
4.Peking Univ, Biomed Pioneering Innovat Ctr BIOPIC, Beijing Adv Innovat Ctr Genom ICG,Sch Life Sci, Peking Tsinghua Ctr Life Sci,Coll Engn, Beijing 100871, Peoples R China;
5.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China;
6.Univ Calif San Francisco, Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;
7.Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08540 USA
Recommended Citation
GB/T 7714
Yun JL,Zheng XW,Xu P,et al. Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis[J]. SMALL,2019:8.
APA Yun JL.,Zheng XW.,Xu P.,郑旭.,Xu JY.,...&Du WB.(2019).Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis.SMALL,8.
MLA Yun JL,et al."Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis".SMALL (2019):8.
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