Adsorption of Phospholipids at the Air-Water Surface

doi:10.1016/j.bpj.2019.08.022

IMECH-IR > 非线性力学国家重点实验室

	Adsorption of Phospholipids at the Air-Water Surface
	Bai X(白轩)1,2,3 ; Xu L4 ; Tang JY 4; Zuo YY 4,5; Hu GQ(胡国庆)1
通讯作者	Zuo, Yi Y.(yzuo@hawaii.edu) ; Hu, Guoqing(ghu@zju.edu.cn)
发表期刊	BIOPHYSICAL JOURNAL
	2019-10-01
卷号	117 期号:7 页码:1224-1233
ISSN	0006-3495
摘要	Phospholipids are ubiquitous components of biomembranes and common biomaterials used in many bioengineering applications. Understanding adsorption of phospholipids at the air-water surface plays an important role in the study of pulmonary surfactants and cell membranes. To date, however, the biophysical mechanisms of phospholipid adsorption are still unknown. It is challenging to reveal the molecular structure of adsorbed phospholipid films. Using combined experiments with constrained drop surfactometry and molecular dynamics simulations, here, we studied the biophysical mechanisms of dipalmitoylphosphatidylcholine (DPPC) adsorption at the air-water surface. It was found that the DPPC film adsorbed from vesicles showed distinct equilibrium surface tensions from the DPPC monolayer spread via organic solvents. Our simulations revealed that only the outer leaflet of the DPPC vesicle is capable of unzipping and spreading at the air-water surface, whereas the inner leaflet remains intact and forms an inverted micelle to the interfacial monolayer. This inverted micelle increases the local curvature of the monolayer, thus leading to a loosely packed monolayer at the air-water surface and hence a higher equilibrium surface tension. These findings provide novel insights, to our knowledge, into the mechanism of the phospholipid and pulmonary surfactant adsorption and may help understand the structure-function correlation in biomembranes.
DOI	10.1016/j.bpj.2019.08.022
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000488457600006
关键词[WOS]	COARSE-GRAINED MODEL ; PULMONARY SURFACTANT ; LUNG SURFACTANT ; LIPID-BILAYERS ; COMPETITIVE ADSORPTION ; HYDROPHOBIC SURFACES ; TENSION MEASUREMENTS ; MOLECULAR-MECHANISM ; DYNAMIC ADSORPTION ; SP-B
WOS研究方向	Biophysics
WOS类目	Biophysics
资助项目	Natural Science Foundation of China[11832017] ; Natural Science Foundation of China[11572334] ; Natural Science Foundation of China[91543125] ; Chinese Academy of Sciences Key Research Program of Frontier Sciences[QYZDB-SSW-JSC036] ; Chinese Academy of Sciences Strategic Priority Research Program[XDB22040403] ; National Science Foundation[CBET-1604119] ; Hawai'i Community Foundation[18ADVC-90802]
项目资助者	Natural Science Foundation of China ; Chinese Academy of Sciences Key Research Program of Frontier Sciences ; Chinese Academy of Sciences Strategic Priority Research Program ; National Science Foundation ; Hawai'i Community Foundation
论文分区	一类/力学重要期刊
力学所作者排名	1
RpAuthor	Zuo, Yi Y. ; Hu, Guoqing
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/80661
专题	非线性力学国家重点实验室
作者单位	1.Zhejiang Univ, Dept Engn Mech, Hangzhou, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China; 4.Univ Hawaii Manoa, Dept Mech Engn, John A Burns Sch Med, Honolulu, HI 96822 USA; 5.Univ Hawaii Manoa, Dept Pediat, John A Burns Sch Med, Honolulu, HI 96822 USA
推荐引用方式 GB/T 7714	Bai X,Xu L,Tang JY,et al. Adsorption of Phospholipids at the Air-Water Surface[J]. BIOPHYSICAL JOURNAL,2019,117,7,:1224-1233.
APA	白轩,Xu L,Tang JY,Zuo YY,&胡国庆.(2019).Adsorption of Phospholipids at the Air-Water Surface.BIOPHYSICAL JOURNAL,117(7),1224-1233.
MLA	白轩,et al."Adsorption of Phospholipids at the Air-Water Surface".BIOPHYSICAL JOURNAL 117.7(2019):1224-1233.

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