Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization

doi:10.1016/j.bpj.2019.09.012

IMECH-IR > 微重力重点实验室

	Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization
	Hu JR(胡锦荣)1,2,3 ; Chen SB(陈深宝)1,2,3 ; Hu WH(胡文慧)1,2,4; Lv SQ(吕守芹)1,2,3 ; Long M(龙勉)1,2,3
通讯作者	Lu, Shouqin(lsq@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn)
发表期刊	BIOPHYSICAL JOURNAL
	2019-10-15
卷号	117 期号:8 页码:1405-1418
ISSN	0006-3495
摘要	Global cytoskeleton reorganization is well-recognized when cells are exposed to distinct mechanical stimuli, but the localized responses at a specified region of a cell are still unclear. In this work, we mapped the cell-surface mechanical property of single cells in situ before and after static point loading these cells using atomic force microscopy in PeakForce-Quantitative Nano Mechanics mode. Cell-surface stiffness was elevated at a maximum of 1.35-fold at the vicinity of loading site, indicating an enhanced structural protection of the cortex to the cell. Mechanical modeling also elucidated the structural protection from the stiffened cell cortex, in which 9-15% and 10-19% decrease of maximum stress and strain of the nucleus were obtained. Furthermore, the flat-ended atomic force microscopy probes were used to capture cytoskeleton reorganization after point loading quantitatively, revealing that the larger the applied force and the longer the loading time are, the more pronounced cytoskeleton reorganization is. Also, point loading using a microneedle combined with real-time confocal microscopy uncovered the fast dynamics of actin cytoskeleton reorganization for actin-stained live cells after point loading (<10 s). These results furthered the understandings in the transmission of localized mechanical forces into an adherent cell.
DOI	10.1016/j.bpj.2019.09.012
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000490144100004
关键词[WOS]	ATOMIC-FORCE MICROSCOPY ; INDUCED CYTOSKELETAL REORGANIZATION ; CELL STIFFNESS ; CYTOCHALASIN-D ; ENDOTHELIAL-CELLS ; PLASMA-MEMBRANE ; CANCER-CELLS ; STRESS ; DECONDENSATION ; ORGANIZATION
WOS研究方向	Biophysics
WOS类目	Biophysics
资助项目	National Key Research and Development Program of China[2016YFA0501601] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31570942] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB22040101]
项目资助者	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy ; Strategic Priority Research Program of Chinese Academy of Sciences
论文分区	一类/力学重要期刊
力学所作者排名	1
RpAuthor	Lu, Shouqin ; Long, Mian
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/80670
专题	微重力重点实验室
作者单位	1.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab,Beijing Key, Beijing, Peoples R China; 2.Chinese Acad Sci, CAS Ctr Excellence Complex Syst Mech, Inst Mech, Beijing, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China; 4.Guizhou Med Univ, Key Lab Biol & Med Engn, Immune Cells & Antibody Engn Res Ctr Guizhou Prov, Guiyang, Guizhou, Peoples R China
推荐引用方式 GB/T 7714	Hu JR,Chen SB,Hu WH,et al. Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization[J]. BIOPHYSICAL JOURNAL,2019,117,8,:1405-1418.
APA	胡锦荣,陈深宝,胡文慧,吕守芹,&龙勉.(2019).Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization.BIOPHYSICAL JOURNAL,117(8),1405-1418.
MLA	胡锦荣,et al."Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization".BIOPHYSICAL JOURNAL 117.8(2019):1405-1418.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Jp2019397.pdf（4950KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载