IMECH-IR  > 国家微重力实验室
Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization
Hu JR(胡锦荣)1,2,3; Chen SB(陈深宝)1,2,3; Hu WH(胡文慧)1,2,4; Lv SQ(吕守芹)1,2,3; Long M(龙勉)1,2,3
Corresponding AuthorLu, Shouqin(lsq@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn)
Source PublicationBIOPHYSICAL JOURNAL
2019-10-15
Volume117Issue:8Pages:1405-1418
ISSN0006-3495
AbstractGlobal cytoskeleton reorganization is well-recognized when cells are exposed to distinct mechanical stimuli, but the localized responses at a specified region of a cell are still unclear. In this work, we mapped the cell-surface mechanical property of single cells in situ before and after static point loading these cells using atomic force microscopy in PeakForce-Quantitative Nano Mechanics mode. Cell-surface stiffness was elevated at a maximum of 1.35-fold at the vicinity of loading site, indicating an enhanced structural protection of the cortex to the cell. Mechanical modeling also elucidated the structural protection from the stiffened cell cortex, in which 9-15% and 10-19% decrease of maximum stress and strain of the nucleus were obtained. Furthermore, the flat-ended atomic force microscopy probes were used to capture cytoskeleton reorganization after point loading quantitatively, revealing that the larger the applied force and the longer the loading time are, the more pronounced cytoskeleton reorganization is. Also, point loading using a microneedle combined with real-time confocal microscopy uncovered the fast dynamics of actin cytoskeleton reorganization for actin-stained live cells after point loading (<10 s). These results furthered the understandings in the transmission of localized mechanical forces into an adherent cell.
DOI10.1016/j.bpj.2019.09.012
Indexed BySCI ; EI
Language英语
WOS IDWOS:000490144100004
WOS KeywordATOMIC-FORCE MICROSCOPY ; INDUCED CYTOSKELETAL REORGANIZATION ; CELL STIFFNESS ; CYTOCHALASIN-D ; ENDOTHELIAL-CELLS ; PLASMA-MEMBRANE ; CANCER-CELLS ; STRESS ; DECONDENSATION ; ORGANIZATION
WOS Research AreaBiophysics
WOS SubjectBiophysics
Funding ProjectNational Key Research and Development Program of China[2016YFA0501601] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31570942] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB22040101]
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy ; Strategic Priority Research Program of Chinese Academy of Sciences
Classification一类/力学重要期刊
Ranking1
ContributorLu, Shouqin ; Long, Mian
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/80670
Collection国家微重力实验室
Affiliation1.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab,Beijing Key, Beijing, Peoples R China;
2.Chinese Acad Sci, CAS Ctr Excellence Complex Syst Mech, Inst Mech, Beijing, Peoples R China;
3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China;
4.Guizhou Med Univ, Key Lab Biol & Med Engn, Immune Cells & Antibody Engn Res Ctr Guizhou Prov, Guiyang, Guizhou, Peoples R China
Recommended Citation
GB/T 7714
Hu JR,Chen SB,Hu WH,et al. Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization[J]. BIOPHYSICAL JOURNAL,2019,117(8):1405-1418.
APA 胡锦荣,陈深宝,胡文慧,吕守芹,&龙勉.(2019).Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization.BIOPHYSICAL JOURNAL,117(8),1405-1418.
MLA 胡锦荣,et al."Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization".BIOPHYSICAL JOURNAL 117.8(2019):1405-1418.
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