IMECH-IR  > 国家微重力实验室
Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation
Wang JW(王家文)1,2,3; Wu Y(武亿)1,2,3; Zhang X(张潇)1,2,3; Zhang F(张帆)1,2,3; Lv DY(吕东媛)1,2,3; Bing SG1,2; Gao YX(高宇欣)1,2; Long M(龙勉)1,2,3
Corresponding AuthorLong, Mian(mlong@imech.ac.cn)
Source PublicationSTEM CELL RESEARCH & THERAPY
2019-11-27
Volume10Issue:1Pages:16
AbstractBackground Distinct mechanical stimuli are known to manipulate the behaviors of embryonic stem cells (ESCs). Fundamental rationale of how ESCs respond to mechanical forces and the potential biological effects remain elusive. Here we conducted the mechanobiological study for hESCs upon mechanomics analysis to unravel typical mechanosensitive processes on hESC-specific fluid shear. Methods hESC line H1 was subjected to systematically varied shear flow, and mechanosensitive proteins were obtained by mass spectrometry (MS) analysis. Then, function enrichment analysis was performed to identify the enriched gene sets. Under a steady shear flow of 1.1 Pa for 24 h, protein expressions were further detected using western blotting (WB), quantitative real-time PCR (qPCR), and immunofluorescence (IF) staining. Meanwhile, the cells were treated with 200 nM trichostatin (TSA) for 1 h as positive control to test chromatin decondensation. Actin, DNA, and RNA were then visualized with TRITC-labeled phalloidin, Hoechst 33342, and SYTO (R) RNASelect (TM) green fluorescent cell stain (Life Technologies), respectively. In addition, cell stiffness was determined with atomic force microscopy (AFM) and annexin V-PE was used to determine the apoptosis with a flow cytometer (FCM). Results Typical mechanosensitive proteins were unraveled upon mechanomics analysis under fluid shear related to hESCs in vivo. Functional analyses revealed significant alterations in histone acetylation, nuclear size, and cytoskeleton for hESC under shear flow. Shear flow was able to induce H2B acetylation and nuclear spreading by CFL2/F-actin cytoskeletal reorganization. The resulting chromatin decondensation and a larger nucleus readily accommodate signaling molecules and transcription factors. Conclusions Shear flow regulated chromatin dynamics in hESCs via cytoskeleton and nucleus alterations and consolidated their primed state.
KeywordEmbryonic stem cell Fluid shear mechanomics Nuclear spreading Histone acetylation Chromatin decondensation
DOI10.1186/s13287-019-1454-z
Indexed BySCI
Language英语
WOS IDWOS:000499837400001
WOS KeywordEMBRYONIC STEM-CELLS ; EPITHELIAL-MESENCHYMAL TRANSITION ; SHEAR-STRESS ; BIOPHYSICAL REGULATION ; ENDOTHELIAL-CELLS ; HISTONE ACETYLATION ; MECHANICAL CONTROL ; ACTIN DYNAMICS ; SELF-RENEWAL ; FORCES
WOS Research AreaCell Biology ; Research & Experimental Medicine
WOS SubjectCell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental
Funding ProjectNational Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31470907] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018]
Funding OrganizationNational Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy
Classification一类
Ranking1
ContributorLong, Mian
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/81207
Collection国家微重力实验室
Affiliation1.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China;
2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;
3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wang JW,Wu Y,Zhang X,et al. Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation[J]. STEM CELL RESEARCH & THERAPY,2019,10(1):16.
APA 王家文.,武亿.,张潇.,张帆.,吕东媛.,...&龙勉.(2019).Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation.STEM CELL RESEARCH & THERAPY,10(1),16.
MLA 王家文,et al."Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation".STEM CELL RESEARCH & THERAPY 10.1(2019):16.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[王家文]'s Articles
[武亿]'s Articles
[张潇]'s Articles
Baidu academic
Similar articles in Baidu academic
[王家文]'s Articles
[武亿]'s Articles
[张潇]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[王家文]'s Articles
[武亿]'s Articles
[张潇]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.