Cellular fate of deformable needle-shaped PLGA-PEG fibers

doi:10.1016/j.actbio.2020.05.029

IMECH-IR > 非线性力学国家重点实验室

	Cellular fate of deformable needle-shaped PLGA-PEG fibers
	Zhang BK 1,3; Zhu ML(朱鸣柳)2,7 ; Li Z 3,6; Lung PS 1; Chrzanowski W 4; Kwok CT 5; Lu J 2,8; Li Q 1
发表期刊	ACTA BIOMATERIALIA
	2020-08-01
卷号	112 页码:182-189
ISSN	1742-7061
摘要	Deformability of micro/nanometer sized particles plays an important role in particle-cell interactions and thus becomes a key parameter in carrier design in biomedicine application such as drug delivery and vaccinology. Yet the influence of material's deformability on the cellular fate of the particles as well as physiology response of live cells are to be understood. Here we show the cellular fate of needle shaped (high aspect ratio similar to 25) PLGA-PEG copolymer fibers depending on their deformability. We found that all the fibers entered murine macrophage cells (RAW 264.7) via phagocytosis. While the fibers of high apparent Young's modulus (average value = 872 kPa) maintained their original shape upon phagocytosis, their counterparts of low apparent Young's modulus (average value = 56 kPa) curled in cells. The observed deformation of fibers of low apparent Young's modulus in cells coincided with abnormal intracellular actin translocation and absence of lysosome/phagosome fusion in macrophages, suggesting the important role of material mechanical properties and mechano-related cellular pathway in affecting cell physiology. Statement of Significance Particles are increasingly important in the field of biomedicine, especially when they are serving as drug carriers. Physical cues, such as mechanical properties, were shown to provide insight into their stability and influence on physiology inside the cell. In the current study, we managed to fabricate 5 types of needle shaped PLGA-PEG fibers with controlled Young's modulus. We found that hard fibers maintained their original shape upon phagocytosis, while soft fibers were curled by actin compressive force inside the cell, causing abnormal actin translocation and impediment of lysosome/phagosome fusion, suggesting the important role of material mechanical properties and mechano-related cellular pathway in affecting cell physiology. (c) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
关键词	Deformability Apparent Young's modulus Actin revolution
DOI	10.1016/j.actbio.2020.05.029
收录类别	SCI
语种	英语
WOS记录号	WOS:000549500900015
关键词[WOS]	NANOPARTICLE SIZE ; SURFACE-CHARGE ; DRUG-DELIVERY ; ACTIN ; FORCES ; POLYMERIZATION ; REQUIREMENTS ; PHAGOCYTOSIS ; CIRCULATION ; PARTICLES
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
项目资助者	Hong Kong Collaborative Research Fund (CRF) Scheme[C4026-17W] ; Theme-based Research Scheme[T13-402/17-N]
论文分区	一类
力学所作者排名	2
RpAuthor	Lu, Jian ; Li, Quan
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/84738
专题	非线性力学国家重点实验室
作者单位	1.Chinese Univ Hong Kong, Dept Phys, Hong Kong, Peoples R China; 2.City Univ Hong Kong, Dept Mech Engn, Kowloon, Hong Kong, Peoples R China; 3.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Key Lab Nanobiomech, Beijing, Peoples R China; 4.Univ Sydney, Fac Pharm, Sydney, NSW, Australia; 5.Univ Macau, Dept Electromech Engn, Zhuhai, Australia; 6.Nanjing Univ Aeronaut & Astronaut, State Key Lab Mech & Control Mech Struct, Nanjing 210016, Jiangsu, Peoples R China; 7.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, 15 West Rd,North 4th Ring, Beijing 100190, Peoples R China; 8.City Univ Hong Kong, Ctr Adv Struct Mat, Greater Bay Joint Div, Shenyang Natl Lab Mat Sci,Shenzhen Res Inst, Shenzhen 518057, Peoples R China
推荐引用方式 GB/T 7714	Zhang BK,Zhu ML,Li Z,et al. Cellular fate of deformable needle-shaped PLGA-PEG fibers[J]. ACTA BIOMATERIALIA,2020,112:182-189.
APA	Zhang BK.,朱鸣柳.,Li Z.,Lung PS.,Chrzanowski W.,...&Li Q.(2020).Cellular fate of deformable needle-shaped PLGA-PEG fibers.ACTA BIOMATERIALIA,112,182-189.
MLA	Zhang BK,et al."Cellular fate of deformable needle-shaped PLGA-PEG fibers".ACTA BIOMATERIALIA 112(2020):182-189.

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