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Regulation of morphology and differentiation of embryonic stem cells by substrate stiffness and topography
Lv DY(吕东媛)
Source Publication中国生理学会基质生物学专业委员会第四次全国基质生物学学术会议会议手册
2019-06-27
Pages46
Conference Name中国生理学会基质生物学专业委员会第四次全国基质生物学学术会议
Conference Date2019-06-27
Conference Place中国江苏常州
AbstractResults and Discussion:Hepatic differentiation of hESCs on topographical PA hydrogel with two typical stiffnesses was systematically tested using a four-stage induction protocol,we proposed a working model to illustrate how the two mechanical factors cooperatively manipulate the hepatic differentiation of hESCs.Substrate stiffness and topography are critical in regulating cell functions,coupled impacts of topography and stiffness were found differentially at distinct differentiation stages.Substrate stiffness is dominant in sternness maintenance and defined endoderm differentiation.Substrate topography plays a decisive role in manipulating clone or cell morphology and spreading and favors the differentiation to hepatocyte-like cells formation and maturation.Both substrate stiffness and topography cooperatively support clone formation or cell shape maintenance,and the differentiated cells present liver-specific functions,such as albumin excretion,glycogen synthesis,and ICG engulfment.Conclusions:These results indicate that stem cell morphology and differentiation are affected by substrate stiffness and topography that mimics physiological conditions,combined with those biochemical factors.Biomechanical cues are critical for enhancing the efficiency and maturity of hepatic differentiation of ESCs.
KeywordRegulation of morphology and differentiation of embryonic stem cells by substrate stiffness and topography
Language英语
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/84945
Collection国家微重力实验室
Affiliation中国科学院力学研究所
Recommended Citation
GB/T 7714
Lv DY. Regulation of morphology and differentiation of embryonic stem cells by substrate stiffness and topography[C]中国生理学会基质生物学专业委员会第四次全国基质生物学学术会议会议手册,2019:46.
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