Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces | |
Almeida, Luis C.1; Frade, Tania1; Correia, Rui D.1; Niu Y(牛宇)2; Jin G(靳刚)2; Correia, Jorge P.1; Viana, Ana S.1 | |
通讯作者 | Viana, Ana S.(anaviana@fc.ul.pt) |
发表期刊 | SCIENTIFIC REPORTS |
2021-01-26 | |
卷号 | 11期号:1页码:12 |
ISSN | 2045-2322 |
摘要 | We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantage of the electrochemical methods is to generate the oxidized species (quinones), which can readily react with ETA amine groups present in solution, with the subsequent incorporation of this molecule in the polymer. The presence of ETA and its effect on the electrosynthesis of polydopamine was accessed by cyclic voltammetry, ellipsometry, atomic force microscopy, FTIR and X-ray photoelectron spectroscopy. The adhesive and biocompatible films enable a facile protein linkage, are resilient to flow assays, and display intrinsic anti-fouling properties to block non-specific protein interactions, as monitored by real-time surface plasmon resonance, and confirmed by ellipsometry. Immunoglobulin G (IgG) and Anti-IgG were used in this work as model proteins for the affinity sensor. By using the one-step methodology (ePDA-ETA), the lower amount of immobilized biorecognition element, IgG, compared to that deposited on ePDA or on ETA post-modified film (ePDA/ETA), allied to the presence of ETA, improved the antibody-antigen affinity interaction. The great potential of the developed platform is its versatility to be used with any target biorecognition molecules, allowing both optical and electrochemical detection. |
DOI | 10.1038/s41598-021-81816-1 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000667507900024 |
关键词[WOS] | SURFACE-CHEMISTRY ; CARBON-DISULFIDE ; DOPAMINE ; ADSORPTION ; LACCASE ; IMMOBILIZATION ; NANOPARTICLES ; OXIDATION ; OXIDE ; TOOL |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
资助项目 | Fundacao para a Ciencia e Tecnologia (FCT)[UIDB/00100/2020] ; Fundacao para a Ciencia e Tecnologia (FCT)[SFRH/BD/129566/2017] ; Fundacao para a Ciencia e Tecnologia (FCT)[PTDC/CTM-NAN/0994/2014] |
项目资助者 | Fundacao para a Ciencia e Tecnologia (FCT) |
论文分区 | 二类/Q1 |
力学所作者排名 | 3+ |
RpAuthor | Viana, Ana S. |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://dspace.imech.ac.cn/handle/311007/87032 |
专题 | 微重力重点实验室 |
作者单位 | 1.Univ Lisbon, Fac Ciencias, Ctr Quim Estrutural, P-1749016 Lisbon, Portugal; 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, NML, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Almeida, Luis C.,Frade, Tania,Correia, Rui D.,et al. Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces[J]. SCIENTIFIC REPORTS,2021,11,1,:12. |
APA | Almeida, Luis C..,Frade, Tania.,Correia, Rui D..,牛宇.,靳刚.,...&Viana, Ana S..(2021).Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces.SCIENTIFIC REPORTS,11(1),12. |
MLA | Almeida, Luis C.,et al."Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces".SCIENTIFIC REPORTS 11.1(2021):12. |
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