| A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method | |
Qiao, Chunxia1,2; Lv, Ming2,3; Li, Xinying1,2,3; Lang, Xiaoling4; Lv SQ(吕守芹)5 ; Long M(龙勉)5; Li, Yan2,3; Geng, Shusheng4; Lin, Zhou2,3; Shen, Beifen2,3; Feng, Jiannan1,2
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| 通讯作者 | Shen, Beifen(shenbf@mx.cei.gov.cn) ; Feng, Jiannan(fengjn@nic.bmi.ac.cn) |
| 发表期刊 | ENGINEERING
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| 2021-11-01 | |
| 卷号 | 7期号:11页码:1566-1576 |
| ISSN | 2095-8099 |
| 摘要 | Fully human antibodies have minimal immunogenicity and safety profiles. At present, most potential antibody drugs in clinical trials are humanized or fully human. Human antibodies are mostly generated using the phage display method (in vitro) or by transgenic mice (in vivo); other methods include B lymphocyte immortalization, human-human hybridoma, and single-cell polymerase chain reaction. Here, we describe a structure-based computer-aided de novo design technology for human antibody generation. Based on the complex structure of human epidermal growth factor receptor 2 (HER2)/Herceptin, we first designed six short peptides targeting the potential epitope of HER2 recognized by Herceptin. Next, these peptides were set as complementarity determining regions in a suitable immunoglobulin frame, giving birth to a novel anti-HER2 antibody named "HF," which possessed higher affinity and more effective anti-tumor activity than Herceptin. Our work offers a useful tool for the quick design and selection of novel human antibodies for basic mechanical research as well as for imaging and clinical applications in immune-related diseases, such as cancer and infectious diseases. (C) 2021 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. |
| 关键词 | HER2/erb-B2 Human antibody Computer-aided design |
| DOI | 10.1016/j.eng.2020.10.024 |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000738823800011 |
| 关键词[WOS] | TNF-ALPHA ; COMPUTATIONAL DESIGN ; RECEPTOR ; COMPLEX ; REGION ; HER2 |
| WOS研究方向 | Engineering |
| WOS类目 | Engineering, Multidisciplinary |
| 资助项目 | National Sciences Fund[31370938] ; National Sciences Fund[81272528] |
| 项目资助者 | National Sciences Fund |
| 论文分区 | 一类 |
| 力学所作者排名 | 3+ |
| RpAuthor | Shen, Beifen ; Feng, Jiannan |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/88294 |
| 专题 | 微重力重点实验室 |
| 作者单位 | 1.Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Beijing 100850, Peoples R China; 2.Beijing Key Lab Therapeut Gene Engn Antibody, Beijing 100850, Peoples R China; 3.Inst Mil Cognit & Brain Sci, Beijing 100850, Peoples R China; 4.Beijing Mabworks Biotech Co Ltd, Beijing 100176, Peoples R China; 5.Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Chunxia,Lv, Ming,Li, Xinying,et al. A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method[J]. ENGINEERING,2021,7,11,:1566-1576. |
| APA | Qiao, Chunxia.,Lv, Ming.,Li, Xinying.,Lang, Xiaoling.,吕守芹.,...&Feng, Jiannan.(2021).A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method.ENGINEERING,7(11),1566-1576. |
| MLA | Qiao, Chunxia,et al."A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method".ENGINEERING 7.11(2021):1566-1576. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A Novel Human Antibo（4174KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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