| Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip | |
Huang, Chenghong1; Tang, Hong1; Chen, Hongjie1; Huang, Xiaorong1; Fang, Ying1; Wang, KangRui1; Men, Rongrong1; Gao, Jie1; Wang, Yang1; Chen YY(陈艳艳)2 ; Jin G(靳刚)3
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| 通讯作者 | Huang, Chenghong(chhuang2007@sinano.ac.cn) ; Chen, Yanyan(yychen2006@sinano.ac.cn) ; Jin, Gang(gajin@imech.ac.cn) |
| 发表期刊 | INTEGRATED FERROELECTRICS
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| 2022-06-13 | |
| 卷号 | 226期号:1页码:148-155 |
| ISSN | 1058-4587 |
| 摘要 | For immunoassay, immobilization of antibody is the most important technique in bioengineering for maintaining its intrinsic activity of antigen binding. The sugar chain in the Fc region carried by antibody can be oxidized into aldehyde group by sodium m-periodate, which can couple with the amino-group on chemistry modified substrate surface through covalent linking. Thereby, in situ site-directed immobilization of the oxidized antibody for only one-step reaction can be performed. Here we propose a facile method that oxidized anti-human alpha-fetoprotein antibody (anti-AFP) was in situ site-directed immobilized on APTES treated silicon substrate utilizing microfluidic channel system for AFP immunoassay. The shape of the immobilized anti-AFP was observed by AFM and imaged by imaging ellipsometry of self-developed protein system. Results shown that the amount of antibody immobilization amount and capacity of antigen binding of random immobilization and site-directed immobilization were separately enhanced 16.0% and 7.0%. the suggested strategy has the advantages of simple, environment-friend and convenient-operation. |
| 关键词 | protein chip alpha-fetoprotein site-directed immobilization of antibody |
| DOI | 10.1080/10584587.2022.2061203 |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000805668600015 |
| 关键词[WOS] | ORIENTATION ; STRATEGY |
| WOS研究方向 | Engineering ; Physics |
| WOS类目 | Engineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter |
| 资助项目 | NSF of Chongqing[cstc2019jscx-msxm0366] ; Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism[GYFJWSW-201905] ; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission[KJZD-K201806401] ; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission[KJZD-K201906401] ; graduate Innovation Fund of CQUST[YKJCX2020509] ; graduate Innovation Fund of CQUST[YKJCX2120502] ; graduate Innovation Fund of CQUST[YKJCX2120541] ; graduate Innovation Fund of CQUST[202111551006] |
| 项目资助者 | NSF of Chongqing ; Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism ; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission ; graduate Innovation Fund of CQUST |
| 论文分区 | Q4 |
| 力学所作者排名 | 1 |
| RpAuthor | Huang, Chenghong ; Chen, Yanyan ; Jin, Gang |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/89637 |
| 专题 | 微重力重点实验室 |
| 作者单位 | 1.CQUST, Chongqing Key Lab Ind Fermentat Microorganism, Chongqing, Peoples R China; 2.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China; 3.Chinese Acad Sci, Inst Mech, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Chenghong,Tang, Hong,Chen, Hongjie,et al. Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip[J]. INTEGRATED FERROELECTRICS,2022,226,1,:148-155. |
| APA | Huang, Chenghong.,Tang, Hong.,Chen, Hongjie.,Huang, Xiaorong.,Fang, Ying.,...&靳刚.(2022).Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip.INTEGRATED FERROELECTRICS,226(1),148-155. |
| MLA | Huang, Chenghong,et al."Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip".INTEGRATED FERROELECTRICS 226.1(2022):148-155. |
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