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Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry
Wang ZH(王战会); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
2004
发表期刊Journal of Immunological Methods
卷号285期号:2页码:237-243
ISSN0022-1759
摘要In the development of biosensors, the immobilization of biomolecules at interfaces played a crucial role. The feasibility of using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify silicon surface to immobilize covalently protein for immunoassay with the biosensor based on imaging ellipsometry was investigated. The higher density and stability of human IgG layer could be obtained on the silicon surface modified with APTES and Glu than that on the silicon surface modified with dichlorodimethylsilane (DIDS). The human IgG molecules immobilized covalently on APTES-Glu surface bound more anti-IgG molecules than that on DDS surface, which indicated that the human IgG molecules could maintain higher binding capability on APTES-Glu surface. Tween 20 was able to block the undesirable adsorption on APTES-Glu surface, and also enhanced the recognition between human IgG and its antibody on both APTES-Glu and DDS surfaces. The combination of this protein covalent immobilization and the biosensor has the potential to be developed into a fast, simple immunoassay technique.
学科领域力学
DOI10.1016/j.jim.2003.12.002
收录类别SCI
语种英语
WOS记录号WOS:000220140200009
关键词[WOS]SILICA SURFACES ; VISUALIZATION ; ANTIBODY ; INTERFACES ; PLASMA
WOS研究方向Biochemistry & Molecular Biology ; Immunology
WOS类目Biochemical Research Methods ; Immunology
引用统计
被引频次:77[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/15439
专题力学所知识产出(1956-2008)
通讯作者Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
推荐引用方式
GB/T 7714
Wang ZH,Jin G,Jin, G . Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry[J]. Journal of Immunological Methods,2004,285(2):237-243.
APA 王战会,靳刚,&Jin, G .(2004).Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry.Journal of Immunological Methods,285(2),237-243.
MLA 王战会,et al."Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry".Journal of Immunological Methods 285.2(2004):237-243.
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