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Title:
Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry
Author: Wang ZH(王战会); Jin G(靳刚)
Source: Journal of Immunological Methods
Issued Date: 2004
Volume: 285, Issue:2, Pages:237-243
Abstract: In the development of biosensors, the immobilization of biomolecules at interfaces played a crucial role. The feasibility of using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify silicon surface to immobilize covalently protein for immunoassay with the biosensor based on imaging ellipsometry was investigated. The higher density and stability of human IgG layer could be obtained on the silicon surface modified with APTES and Glu than that on the silicon surface modified with dichlorodimethylsilane (DIDS). The human IgG molecules immobilized covalently on APTES-Glu surface bound more anti-IgG molecules than that on DDS surface, which indicated that the human IgG molecules could maintain higher binding capability on APTES-Glu surface. Tween 20 was able to block the undesirable adsorption on APTES-Glu surface, and also enhanced the recognition between human IgG and its antibody on both APTES-Glu and DDS surfaces. The combination of this protein covalent immobilization and the biosensor has the potential to be developed into a fast, simple immunoassay technique.
Language: 英语
Indexed Type: SCI
Corresponding Author: Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
Correspondent Email: gajin@imech.ac.cn
DOI: 10.1016/j.jim.2003.12.002
DOC Type: Article
WOS Subject: Biochemical Research Methods ; Immunology
WOS Subject Extended: Biochemistry & Molecular Biology ; Immunology
WOS Keyword Plus: SILICA SURFACES ; VISUALIZATION ; ANTIBODY ; INTERFACES ; PLASMA
WOS ID: WOS:000220140200009
ISSN: 0022-1759
Subject: 力学
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/15439
Appears in Collections:力学所知识产出(1956-2008)_期刊论文

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Recommended Citation:
Wang ZH,Jin G. Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry[J]. Journal of Immunological Methods,2004-01-01,285(2):237-243.
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